Sandikapura Muhammad Jihad, Nyamathulla Shaik, Noordin Mohamed Ibrahim
Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia / Centre for Natural Products Research and Drug Discovery (CENAR), Department of Chemistry, Faculty of Science, Kuala Lumpur, Malaysia.
Pak J Pharm Sci. 2018 Mar;31(2(Suppl.)):623-635.
The study was aimed to perform aqueous extraction of two plants using different extraction methods, and evaluate their antioxidant and antidiabetic potential. Plant materials were extracted by maceration, soxhlet, sonication and fresh juice methods to produce aqueous extracts. In vitro antioxidant DPPH (1,1-diphenyl-2- picrylhydrazyl) and FRAP (Ferric reducing antioxidant power), antidiabetic α-amylase and α-glucosidase enzyme inhibitory assays were carried out on the extracts. Extracts of Syzygium polyanthum demonstrated better free radical scavenging and antidiabetic activity than Momordica charantia. It was observed that the % inhibition of DPPH by fresh juice of S. polyanthum was 64.93 similar to quercetin 69.21 (p>0.05). Its FRAP value (69.05) was significantly (p<0.05) higher than Quercetin (63.27). Its fresh juice alsodemonstrated significant inhibitory actions (p<0.05) against α-amylase (92.21%) and α-glucosidase (96.06%) than acarbose. It is concluded that extracts had varied results due to differences in their chemical composition as noticed in LC-MS. The fresh juice of S. polyanthum has superior in vitro antioxidant and antidiabetic activities. Therefore, intake of exogenous antioxidants in the form of fresh juices of someherbs can help the body toscavenge free radicals and exert hyperglycaemic control in post prandial hyperglycaemia.
本研究旨在采用不同提取方法对两种植物进行水提取，并评估其抗氧化和抗糖尿病潜力。采用浸渍法、索氏提取法、超声提取法和鲜榨法对植物材料进行提取以制备水提取物。对提取物进行体外抗氧化DPPH（1,1-二苯基-2-苦基肼）和FRAP（铁还原抗氧化能力）、抗糖尿病α-淀粉酶和α-葡萄糖苷酶抑制试验。蒲桃提取物比苦瓜提取物表现出更好的自由基清除和抗糖尿病活性。观察到蒲桃鲜榨汁对DPPH的抑制率为64.93，与槲皮素的69.21相似（p>0.05）。其FRAP值（69.05）显著高于槲皮素（63.27）（p<0.05）。其鲜榨汁对α-淀粉酶（92.21%）和α-葡萄糖苷酶（96.06%）的抑制作用也显著高于阿卡波糖（p<0.05）。结论是，如液相色谱-质谱分析所示，由于提取物化学成分的差异，结果各不相同。蒲桃鲜榨汁具有优异的体外抗氧化和抗糖尿病活性。因此，以某些草药鲜榨汁的形式摄入外源性抗氧化剂有助于身体清除自由基并控制餐后高血糖症中的高血糖。
