College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun, Jilin 130062, China.
College of Veterinary Medicine, Jilin University, No. 5333 Xi'an Road, Changchun, Jilin 130062, China.
Microb Pathog. 2018 Jun;119:54-59. doi: 10.1016/j.micpath.2018.04.007. Epub 2018 Apr 6.
The study aimed to investigate whether rutin affects the quorum sensing (QS) of avian pathogenic Escherichia coli (APEC). In this study, APEC-O78 was selected as the test strain. We mainly examined the effects of rutin on the AI-2 secretion by bioluminescence assay, biofilm formation through a crystal violet staining method, and expression of virulence genes of APEC by qRT-PCR. We found that rutin can significantly interfering with QS through reducing the secretion of AI-2, inhibited the biofilm formation, and reduced the expression of virulence genes of APEC. Moreover, rutin markedly decreased adhesion and damage of APEC to chicken type II pneumocytes. These results suggested rutin reduces cell damage of APEC-infected chicken type II pneumocytes through interfering with QS via decreasing AI-2 production, biofilm formation, and the expression of virulence genes. This paper may provide a new evidence for colibacillosis prevention in chicken.
本研究旨在探讨芦丁是否会影响禽致病性大肠杆菌（APEC）的群体感应（QS）。在本研究中，选择 APEC-O78 作为试验菌株。我们主要通过生物发光法检测芦丁对 AI-2 分泌的影响，结晶紫染色法检测生物膜形成，qRT-PCR 检测 APEC 毒力基因的表达。结果发现，芦丁通过减少 AI-2 的分泌，显著干扰 QS，抑制生物膜形成，降低 APEC 毒力基因的表达。此外，芦丁显著降低了 APEC 对鸡Ⅱ型肺泡细胞的黏附和损伤。这些结果表明，芦丁通过降低 AI-2 的产生、生物膜形成和毒力基因的表达，干扰 QS，从而减少 APEC 感染鸡Ⅱ型肺泡细胞的细胞损伤。本研究为鸡大肠杆菌病的防治提供了新的依据。
