Department of Obstetrics, Jinan Maternity and Child Care Hospital, Jinan, Shandong, China.
Eur Rev Med Pharmacol Sci. 2018 Mar;22(6):1549-1553. doi: 10.26355/eurrev_201803_14559.
Polycystic ovary syndrome (PCOS) causes ovulation disorder and infertility in female patients. This study recruited PCOS patients and adopted testosterone therapy to analyze its effect on expression of nuclear peroxisome proliferation activated receptor γ (PPARγ), and cytochrome P450 aromatase (P450arom) in ovary granular cells of patients.
PCOS and non-PCOS patients were treated with testosterone at 1.0 nmol/L, 10 nmol/L. qRT-PCR was used to detect the expressions of PPARγ and P450arom while Western blot was performed for the evaluation of protein expression.
In the experimental group, the level of PPARγ mRNA was significantly elevated whilst the expression of P450arom was statistically decreased (p < 0.05). The treatment of 10 nmol/l testosterone significantly elevated PPARγ mRNA and protein expression, and decreased P450arom expression (p < 0.05). The level of PPARγ was positively correlated with testosterone concentration, and was negatively correlated with P450arom expression (p < 0.05).
Testosterone causes hyperandrogenism microenvironment of PCOS patients, with a correlated increase of PPARγ and reduction of P450arom.
多囊卵巢综合征（PCOS）导致女性患者排卵障碍和不孕。本研究招募了 PCOS 患者，并采用睾酮治疗来分析其对患者卵巢颗粒细胞中核过氧化物酶体增殖物激活受体 γ（PPARγ）和细胞色素 P450 芳香酶（P450arom）表达的影响。
将 PCOS 和非 PCOS 患者分别以 1.0 nmol/L 和 10 nmol/L 的浓度用睾酮处理。采用 qRT-PCR 检测 PPARγ 和 P450arom 的表达，并用 Western blot 评估蛋白表达。
实验组中，PPARγ mRNA 水平显著升高，而 P450arom 的表达则统计学降低（p<0.05）。10 nmol/L 睾酮治疗可显著升高 PPARγ mRNA 和蛋白表达，同时降低 P450arom 表达（p<0.05）。PPARγ 水平与睾酮浓度呈正相关，与 P450arom 表达呈负相关（p<0.05）。
睾酮导致 PCOS 患者出现高雄激素血症微环境，同时伴有 PPARγ 升高和 P450arom 减少。
