Cao Jinghe, Maowulieti Gulijianati, Yu Tao
Department of Reproductive Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China.
Department of Gynecology, Xinjiang Autonomous Region Municipal People's Hospital, Wulumuqi, Xinjiang 830001, P.R. China.
Exp Ther Med. 2019 Mar;17(3):1761-1765. doi: 10.3892/etm.2018.7101. Epub 2018 Dec 14.
Expression of peroxisome proliferator-activated receptor γ (PPARγ) mRNA in ovarian granulosa cells of patients with polycystic ovary syndrome (PCOS) were explored. Ovarian granulosa cells were extracted from 5 patients with PCOS and 30 normal controls. Expression of PPARγ mRNA in granulosa cells of the 5 PCOS patients (observation group) and 5 normal controls (control group) was detected by RT-qPCR. The remaining 25 cases of normal human ovarian granulosa cells were cultured for 48 h, followed by cell culture for another 24 h with different concentrations of testosterone, insulin (INS), and rosiglitazone (RGZ). After that, expression of PPARγ mRNA was detected by RT-qPCR. Relative expression level of PPARγ mRNA in the observation group was significantly lower than that in the control group. Compared with testosterone concentration at 10-2 mol/ml, testosterone concentration at 10-3 mol/ml significantly reduced the expression level of PPARγ mRNA. When the INS concentration was 10-5 mol/ml, relative expression level of PPARγ mRNA was significantly higher than that of the control group (P<0.01). Relative expression level of PPARγ mRNA was significantly higher than that of the control group when INS concentration was 10-4 mol/ml. When the concentration of RGZ was 10-4 mol/ml, the relative expression level of PPARγ mRNA was significantly higher than that of the control group (P<0.01). When concentration of RGZ was 10-3 mol/ml, expression level of PPARγ mRNA was significantly increased comparing to that under a RGZ concentration of 10-4 mol/ml or that of the control group (P<0.01). Appropriate concentrations of testosterone can inhibit the expression of PPARγ mRNA in ovarian granulosa cells, and certain concentrations of INS and RGZ can induce the expression of PPARγ mRNA in ovarian granulosa cells. Abnormal expression of PPARγ mRNA in ovarian granulosa cells may be related to the mechanism of PCOS.
探讨多囊卵巢综合征（PCOS）患者卵巢颗粒细胞中过氧化物酶体增殖物激活受体γ（PPARγ）mRNA的表达。从5例PCOS患者和30例正常对照中提取卵巢颗粒细胞。采用RT-qPCR检测5例PCOS患者（观察组）和5例正常对照（对照组）颗粒细胞中PPARγ mRNA的表达。将其余25例正常人卵巢颗粒细胞培养48 h，然后用不同浓度的睾酮、胰岛素（INS）和罗格列酮（RGZ）再培养24 h。之后，采用RT-qPCR检测PPARγ mRNA的表达。观察组PPARγ mRNA的相对表达水平显著低于对照组。与10-2 mol/ml睾酮浓度相比，10-3 mol/ml睾酮浓度显著降低了PPARγ mRNA的表达水平。当INS浓度为10-5 mol/ml时，PPARγ mRNA的相对表达水平显著高于对照组（P<0.01）。当INS浓度为10-4 mol/ml时，PPARγ mRNA的相对表达水平显著高于对照组。当RGZ浓度为10-4 mol/ml时，PPARγ mRNA的相对表达水平显著高于对照组（P<0.01）。当RGZ浓度为10-3 mol/ml时，与10-4 mol/ml RGZ浓度或对照组相比，PPARγ mRNA的表达水平显著升高（P<0.01）。适当浓度的睾酮可抑制卵巢颗粒细胞中PPARγ mRNA的表达，一定浓度的INS和RGZ可诱导卵巢颗粒细胞中PPARγ mRNA的表达。卵巢颗粒细胞中PPARγ mRNA的异常表达可能与PCOS的发病机制有关。
