School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi 710055, PR China; Institute of Environmental Microbial Technology, Xi'an University of Architecture and Technology, Xi'an, Shaanxi 710055, PR China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an, Shaanxi 710055, PR China; Institute of Environmental Microbial Technology, Xi'an University of Architecture and Technology, Xi'an, Shaanxi 710055, PR China.
Bioresour Technol. 2018 Jul;260:321-328. doi: 10.1016/j.biortech.2018.03.123. Epub 2018 Mar 31.
A novel strain KS293 exhibiting excellent aerobic and anaerobic denitrification performance was isolated and identified as Paracoccus versutus KS293. Nitrate nitrogen and total organic carbon could be effectively removed by P. versutus KS293 without nitrite accumulation, whilst 82% and 85% of total nitrogen was converted into gaseous products under aerobic and anaerobic conditions (P > .05), respectively. Based on the ratio of anaerobic to aerobic, relative abundance values were increased 1.41, 1.45, and 2.31 folds for nirS, nosZ, and narG, respectively. A comparison of the two-dimensional gel electrophoresis and principal component analysis showed significant differences in proteomic profiles between aerobic and anaerobic conditions. In total, 78 proteins that displayed fluctuations in relative expression were observed. 10 proteins including nitrate reductase, maintenance of cell membrane (TolA), and RNA polymerase-binding transcription factor (DksA) were differentially expressed. These findings demonstrated that P. versutus KS293 was effective for nitrogen removal under aerobic or anaerobic conditions.
一株具有良好好氧及缺氧反硝化性能的新型KS293 菌株被分离并鉴定为 Paracoccus versutus KS293。P. versutus KS293 可在无亚硝酸盐积累的情况下有效去除硝酸盐氮和总有机碳，而在好氧和缺氧条件下，分别有 82%和 85%的总氮转化为气态产物（P>.05）。基于厌氧与好氧的比例，nirS、nosZ 和 narG 的相对丰度值分别增加了 1.41、1.45 和 2.31 倍。二维凝胶电泳和主成分分析的比较表明，好氧和缺氧条件下的蛋白质组图谱存在显著差异。总共观察到 78 个相对表达波动的蛋白质。10 种蛋白质，包括硝酸盐还原酶、细胞膜维持（TolA）和 RNA 聚合酶结合转录因子（DksA），表达水平存在差异。这些发现表明 P. versutus KS293 可有效去除好氧或缺氧条件下的氮。
