State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2018 May;256:366-373. doi: 10.1016/j.biortech.2018.02.049. Epub 2018 Feb 13.
A novel heterotrophic nitrification-aerobic denitrification bacterium, identified as Janthinobacterium sp. M-11, was isolated from the Songhua River. When the initial ammonium concentration was 5 mg·L, 98% of ammonium was removed under cold condition (2 °C) with the C/N ratio of 5 at initial pH 7 and aerobic condition, which demonstrated the significant ammonium removal capacity of M-11 with low nutrient consumption at cold temperature. Denitrification processes under aerobic and anaerobic conditions were also investigated. 89% of nitrite and 89% of nitrate were removed under aerobic condition. Under anaerobic condition, 93% of nitrite and 98% of nitrate were removed. Interestingly, a high amount of nitrite accumulation was observed in the mid-stage of anaerobic denitrification for nitrate. This special phenomenon was probably because of the existence of narG gene amplified in the strain M-11, which would encode membrane-bound nitrate reductase and accelerate the nitrate conversion rate of M-11 under anaerobic condition.
一株新型异养硝化好氧反硝化细菌，被鉴定为交替单胞菌 M-11，从松花江中分离得到。当初始氨氮浓度为 5mg·L 时，在初始 pH 值为 7、好氧条件和 2°C 的低温条件下，C/N 比为 5 时，98%的氨氮被去除，这表明 M-11 在低温下具有低营养消耗的显著氨氮去除能力。还研究了好氧和厌氧条件下的反硝化过程。在好氧条件下，89%的亚硝酸盐和 89%的硝酸盐被去除。在厌氧条件下，93%的亚硝酸盐和 98%的硝酸盐被去除。有趣的是，在硝酸盐厌氧反硝化的中期观察到了大量的亚硝酸盐积累。这种特殊现象可能是由于菌株 M-11 中扩增了 narG 基因，该基因将编码膜结合型硝酸还原酶，并在厌氧条件下加速 M-11 的硝酸盐转化速率。
