IMOD对糖尿病雄性大鼠心脏组织血管生成及表达水平的影响。

Effects of IMOD on angiogenesis, and expression levels in heart tissue of diabetic male rats.

作者信息

Ghaffari-Nasab Arshad, Mirzaie Bavil Fariba, Ghiasi Rafigheh, Sadigh-Eteghad Saeed, Alipour Mohammad Reza

机构信息

Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Avicenna J Phytomed. 2018 Mar-Apr;8(2):152-160.

PMID:29632846

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5885329/

Abstract

OBJECTIVE

Diabetes is associated with vascular complications and impaired angiogenesis. Since angiogenesis plays a crucial role in vascular homeostasis in ischemic heart diseases, in this study, the effect of IMOD™ on and expression level which are altered in impaired angiogenesis were investigated in heart tissue of diabetic rats.

MATERIALS AND METHODS

Forty male Wistar rats (200-250 g) were randomly classified into 4 groups: control (C), IMOD™ (I), diabetes (D), and diabetes+IMOD™ (D+I). For induction of experimental diabetes in animals, a single dose of streptozotocin (STZ; 60mg/kg) was injected intraperitoneally. After 8 weeks of treatment with IMOD™ (20 mg/kg/day), heart tissue samples were removed and used for measurement of and expression level as well as histological studies.

RESULTS

Results of this study showed that diabetes decreased heart tissue angiogenesis which was associated with increased and reduced expression levels (p<0.05) and IMOD™ could reduce the expression of and increase the expression of (p<0.05). Moreover, IMOD™ extensively induced angiogenesis in the heart tissue of diabetic group. However, IMOD™ had no significant effect on expressions of and CDC25, or angiogenesis in healthy rats.

CONCLUSION

This study showed that IMOD™ is able to increase angiogenesis in the heart tissue of diabetic rats. The angiogenic effect of IMOD™ is associated with reduction of expression and increased expression of .

摘要

目的

糖尿病与血管并发症及血管生成受损有关。由于血管生成在缺血性心脏病的血管稳态中起关键作用，因此在本研究中，在糖尿病大鼠的心脏组织中研究了IMOD™对血管生成受损时改变的[具体物质1]和[具体物质2]表达水平的影响。

材料与方法

40只雄性Wistar大鼠（200 - 250克）随机分为4组：对照组（C）、IMOD™组（I）、糖尿病组（D）和糖尿病 + IMOD™组（D + I）。为诱导动物实验性糖尿病，腹腔注射单剂量链脲佐菌素（STZ；60毫克/千克）。用IMOD™（20毫克/千克/天）治疗8周后，取出心脏组织样本，用于测量[具体物质1]和[具体物质2]的表达水平以及进行组织学研究。

结果

本研究结果表明，糖尿病降低了心脏组织血管生成，这与[具体物质1]表达增加和[具体物质2]表达降低有关（p < 0.05），并且IMOD™可降低[具体物质1]的表达并增加[具体物质2]的表达（p < 0.05）。此外，IMOD™在糖尿病组心脏组织中广泛诱导血管生成。然而，IMOD™对健康大鼠的[具体物质3]和细胞周期蛋白依赖性激酶25（CDC25）表达或血管生成没有显著影响。