1 Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Víctor Lamas 1290, 4030000 Concepción, Chile.
2 Departamento de Farmacia, Facultad de Farmacia, Universidad de Concepción, Víctor Lamas 1290, 4030000 Concepción, Chile.
Benef Microbes. 2018 Jun 15;9(4):625-627. doi: 10.3920/BM2017.0160. Epub 2018 Apr 10.
Searching for bacterial probiotics active upon Helicobacter pylori continue to be an important clinical challenge because of the increased prevalence of this highly priority pathogen in humans. In this work, we assess the in vivo anti-H. pylori SS1 (cagA+/vacAs2m2+) properties of a previously isolated human gastric probiotic strain Lactobacillus fermentum UCO-979C by using a Meriones unguiculatus (Mongolian gerbil) model. Animals were administered with a saline suspension of L. fermentum UCO-979C or H. pylori SS1 as negative and positive control for H. pylori colonisation controls, prior to assayed the challenge group that was administered with these two species per animal for detecting protective activity of the probiotic strain against colonisation. The results showed that L. fermentum UCO-979C strongly inhibited the colonisation of H. pylori decreasing up to 87% of the colonisation in the antrum by the pathogen, suggesting that this probiotic strain has a strong probiotic activity against H. pylori in the most valuable animal model for in vivo assays nowadays.
由于人类中这种高度优先病原体的流行率增加，寻找对幽门螺杆菌有活性的细菌益生菌仍然是一个重要的临床挑战。在这项工作中，我们使用 Meriones unguiculatus（蒙古沙鼠）模型评估了先前分离的人类胃益生菌菌株乳酸杆菌 UCO-979C 对 Helicobacter pylori SS1（cagA+/vacAs2m2+）的体内抗 Helicobacter pylori SS1（cagA+/vacAs2m2+）特性。在对动物进行实验之前，用乳酸杆菌 UCO-979C 的生理盐水悬浮液或 H. pylori SS1 作为 H. pylori 定植对照的阴性和阳性对照，然后对接受这两种物质的实验组进行检测，以检测益生菌菌株对定植的保护活性。结果表明，乳酸杆菌 UCO-979C 强烈抑制了 H. pylori 的定植，使病原体在胃窦中的定植减少了 87%，这表明该益生菌菌株对目前最有价值的体内试验动物模型中的 H. pylori 具有很强的益生菌活性。
