Clinic for Infectious Diseases, Clinical Center University of Sarajevo, Bosnia-Herzegovina.
Clinic for Orthopaedic, Clinical Center University of Sarajevo, Bosnia-Herzegovina.
Epidemiol Infect. 2018 May;146(7):854-857. doi: 10.1017/S0950268818000717. Epub 2018 Apr 10.
Granulysin is a recently discovered cytolytic protein of natural killer (NK) cells and cytotoxic T lymphocytes. Studies of healthy and immunocompromised patients with primary or recurrent varicella-zoster infections demonstrate the importance of virus-specific cellular immunity in controlling viral replication, but also some studies presented granulysin as a molecule that can play a role in chickenpox immunopathogenesis. This study investigated possible correlation between serum granulysin levels and clinical course of chickenpox. A total of 69 patients with chickenpox were included in the study. We measured the levels of granulysin and percentage count for CD4+, CD8+ and NK cells in serum for all patients and healthy controls. For detection and quantification of granulysin in sera, we performed ELISA test and flow cytometry for detection, identification and percentage measurement of T and B lymphocytes. Descriptive methods, analysis of variance and multivariate logistic regression were used for statistical data analysis. We found respective correlation between serum granulysin level and severity of clinical presentation. These findings can be a good input for further studies, since there is no relevant prognostic parameter of chickenpox in everyday clinical practice. Granulysin, as a therapeutic, also deserves to be a point of interests in the future. If we prove its potential to stop dissemination of human herpes viruses, possibilities to use it in some life-threatening forms of viral disease can be very valuable.
颗粒酶是自然杀伤 (NK) 细胞和细胞毒性 T 淋巴细胞中一种新发现的细胞溶素蛋白。对原发性或复发性水痘带状疱疹感染的健康和免疫功能低下患者的研究表明，病毒特异性细胞免疫在控制病毒复制方面的重要性，但也有一些研究将颗粒酶视为一种可能在水痘发病机制中发挥作用的分子。本研究探讨了血清颗粒酶水平与水痘临床病程之间的可能相关性。共纳入 69 例水痘患者。我们测量了所有患者和健康对照组血清中颗粒酶水平和 CD4+、CD8+和 NK 细胞的百分比计数。为了检测和定量血清中的颗粒酶，我们进行了 ELISA 试验和流式细胞术，以检测、鉴定和测量 T 和 B 淋巴细胞的百分比。采用描述性方法、方差分析和多变量逻辑回归进行统计数据分析。我们发现血清颗粒酶水平与临床表现的严重程度之间存在相关性。这些发现可以为进一步的研究提供很好的依据，因为在日常临床实践中，水痘没有相关的预后参数。作为一种治疗方法，颗粒酶也值得在未来引起关注。如果我们证明它有潜力阻止人类疱疹病毒的传播，那么在某些危及生命的病毒性疾病中使用它的可能性将非常有价值。
