Zhang Xiumin, Matsuo Kaori, Farmawati Arta, Higashi Yohei, Ogawa Kazuyuki, Nagata Kinya, Nagatomi Ryoichi
Department of Medicine and Science in Sports and Exercise, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 2006 Oct;210(2):117-24. doi: 10.1620/tjem.210.117.
The circulating number of natural killer (NK) cells largely changes after an acute bout of physical exercise. Granulysin is a cytolytic granule protein with a broad range of antimicrobial and tumoricidal activities produced and released by human NK cells and cytolytic T lymphocytes. Since NK cells constitutively produce granulysin, most serum granulysin in healthy humans is derived from NK cells. Serum graulysin levels in the healthy humans may therefore reflect the size of whole-body NK cell population in the body. The aim of this study was to determine the effect of an acute bout of exhaustive exercise on serum granulysin in comparison with the circulating number of NK cells. Six healthy, young male volunteers participated in the study. Each subject underwent both exhaustive exercise and resting sessions in a random order with at least a seven-day interval. Subjects were asked to run to exhaustion on a treadmill with an incremental graded protocol. Blood samples were collected before, immediately after, and 1 hr, 3 hr, 6 hr, 12 hr and 24 hr after exercise. Serum granulysin levels were measured by enzyme-linked immunosorbent assay (ELISA). NK cells were determined by flow cytometry. Exhaustive exercise induced a 4.8-fold increase in peripheral blood NK cells, but no significant change in serum granulysin. Our results support the hypothesis that exhaustive exercise-induced changes in the circulating number of NK cells represent a redistribution of lymphocytes, rather than the change in the size of whole-body NK cell population.
一次剧烈的体育锻炼后，自然杀伤（NK）细胞的循环数量会大幅改变。颗粒溶素是一种具有广泛抗菌和杀肿瘤活性的溶细胞颗粒蛋白，由人类NK细胞和溶细胞性T淋巴细胞产生并释放。由于NK细胞持续产生颗粒溶素，健康人体内的大多数血清颗粒溶素都来源于NK细胞。因此，健康人体内的血清颗粒溶素水平可能反映体内全身NK细胞群体的大小。本研究的目的是确定一次剧烈的力竭性运动对血清颗粒溶素的影响，并与NK细胞的循环数量进行比较。六名健康的年轻男性志愿者参与了该研究。每个受试者以随机顺序进行力竭性运动和休息环节，间隔至少七天。受试者被要求在跑步机上按照递增分级方案跑步至力竭。在运动前、运动后即刻、运动后1小时、3小时、6小时、12小时和24小时采集血样。通过酶联免疫吸附测定（ELISA）测量血清颗粒溶素水平。通过流式细胞术测定NK细胞。力竭性运动导致外周血NK细胞增加4.8倍，但血清颗粒溶素无显著变化。我们的结果支持这样的假设，即力竭性运动引起的NK细胞循环数量变化代表淋巴细胞的重新分布，而非全身NK细胞群体大小的改变。
