Zardi L, Cianfriglia M, Balza E, Carnemolla B, Siri A, Croce C M
EMBO J. 1982;1(8):929-33. doi: 10.1002/j.1460-2075.1982.tb01273.x.
Using three different species-specific monoclonal antibodies we have studied, in human-mouse and human-hamster somatic cell hybrids, the correlation between the presence of different human chromosomes and the ability to release human fibronectin into the tissue culture medium. Presence of human fibronectin was determined by an affinity-radioimmunoassay. In addition, tissue culture media of the different hybrids were separated on SDS-polyacrylamide gels, the proteins were blotted onto a nitrocellulose sheet and human fibronectin visualized by an immunoenzymatic technique. Karyology and determination of isoenzyme markers of specific human chromosomes show that the ability to produce human fibronectin segregated with the presence of human chromosome 2.
我们使用三种不同的物种特异性单克隆抗体，在人-鼠和人-仓鼠体细胞杂种中研究了不同人类染色体的存在与向组织培养基中释放人纤连蛋白能力之间的相关性。人纤连蛋白的存在通过亲和放射免疫测定法确定。此外，将不同杂种的组织培养基在SDS-聚丙烯酰胺凝胶上进行分离，将蛋白质印迹到硝酸纤维素膜上，并通过免疫酶技术检测人纤连蛋白。特定人类染色体的核型分析和同工酶标记物的测定表明，产生人纤连蛋白的能力与人类2号染色体的存在相关。
