• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
The use of pyrosequencing for detection of hemagglutinin mutations associated with increased pathogenicity of H5N1 avian influenza viruses in mammals.焦磷酸测序法用于检测与H5N1禽流感病毒在哺乳动物中致病性增加相关的血凝素突变。
J Vet Diagn Invest. 2018 Jul;30(4):619-622. doi: 10.1177/1040638718769951. Epub 2018 Apr 10.
2
Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response.H5N1流感病毒血凝素蛋白的糖基化通过加剧宿主免疫反应增强其在小鼠中的毒力。
J Virol. 2017 Mar 13;91(7). doi: 10.1128/JVI.02215-16. Print 2017 Apr 1.
3
A single amino acid at the hemagglutinin cleavage site contributes to the pathogenicity and neurovirulence of H5N1 influenza virus in mice.血凝素裂解位点的单个氨基酸有助于 H5N1 流感病毒在小鼠中的致病性和神经毒力。
J Virol. 2012 Jun;86(12):6924-31. doi: 10.1128/JVI.07142-11. Epub 2012 Apr 11.
4
[Three Mutations in the Stalk Region of Hemagglutinin Affect the pH of Fusion and Pathogenicity of H5N1 Influenza Virus].[血凝素茎区的三个突变影响H5N1流感病毒的融合pH值和致病性]
Mol Biol (Mosk). 2018 Nov-Dec;52(6):1029-1037. doi: 10.1134/S0026898418060125.
5
Hemagglutinin glycosylation modulates the pathogenicity and antigenicity of the H5N1 avian influenza virus.血凝素糖基化作用调节H5N1禽流感病毒的致病性和抗原性。
Vet Microbiol. 2015 Feb 25;175(2-4):244-56. doi: 10.1016/j.vetmic.2014.12.011. Epub 2014 Dec 18.
6
The pathogenicity of swan derived H5N1 virus in birds and mammals and its gene analysis.天鹅源H5N1病毒在鸟类和哺乳动物中的致病性及其基因分析。
Virol J. 2014 Nov 29;11:207. doi: 10.1186/s12985-014-0207-y.
7
SUMOylation of Matrix Protein M1 and Filamentous Morphology Collectively Contribute to the Replication and Virulence of Highly Pathogenic H5N1 Avian Influenza Viruses in Mammals.基质蛋白 M1 的 SUMOylation 和丝状形态共同促进高致病性 H5N1 禽流感病毒在哺乳动物中的复制和毒力。
J Virol. 2022 Feb 23;96(4):e0163021. doi: 10.1128/JVI.01630-21. Epub 2021 Dec 15.
8
Molecular determinants within the surface proteins involved in the pathogenicity of H5N1 influenza viruses in chickens.参与H5N1流感病毒对鸡致病性的表面蛋白中的分子决定因素。
J Virol. 2004 Sep;78(18):9954-64. doi: 10.1128/JVI.78.18.9954-9964.2004.
9
T160A mutation-induced deglycosylation at site 158 in hemagglutinin is a critical determinant of the dual receptor binding properties of clade 2.3.4.4 H5NX subtype avian influenza viruses.T160A 突变导致血凝素 158 位糖基化缺失是 2.3.4.4 分支 H5NX 亚型禽流感病毒双重受体结合特性的关键决定因素。
Vet Microbiol. 2018 Apr;217:158-166. doi: 10.1016/j.vetmic.2018.03.018. Epub 2018 Mar 17.
10
Mouse lung-adapted mutation of E190G in hemagglutinin from H5N1 influenza virus contributes to attenuation in mice.H5N1 流感病毒血凝素 E190G 点突变致鼠适应突变株在小鼠体内毒力减弱。
J Med Virol. 2015 Nov;87(11):1816-22. doi: 10.1002/jmv.24257. Epub 2015 Jul 30.

本文引用的文献

1
Evaluation and application of a one-step duplex real-time reverse transcription polymerase chain reaction assay for the rapid detection of influenza A (H7N9) virus from poultry samples.一种用于从家禽样本中快速检测甲型流感（H7N9）病毒的一步双重实时逆转录聚合酶链反应检测法的评估与应用
Arch Virol. 2015 Oct;160(10):2471-7. doi: 10.1007/s00705-015-2511-2. Epub 2015 Jul 16.
2
Epidemiology, Evolution, and Recent Outbreaks of Avian Influenza Virus in China.中国禽流感病毒的流行病学、进化及近期疫情
J Virol. 2015 Sep;89(17):8671-6. doi: 10.1128/JVI.01034-15. Epub 2015 Jun 10.
3
Screening for H7N9 influenza A by matrix gene-based real-time reverse-transcription PCR.基于基质基因的实时逆转录 PCR 筛查 H7N9 流感 A
J Virol Methods. 2014 Jan;195:123-5. doi: 10.1016/j.jviromet.2013.10.016. Epub 2013 Oct 14.
4
Rapid pathotyping of Newcastle Disease Virus by pyrosequencing.焦磷酸测序快速对新城疫病毒进行分型。
J Virol Methods. 2013 Mar;188(1-2):13-20. doi: 10.1016/j.jviromet.2012.11.021. Epub 2012 Nov 23.
5
A single amino acid at the hemagglutinin cleavage site contributes to the pathogenicity and neurovirulence of H5N1 influenza virus in mice.血凝素裂解位点的单个氨基酸有助于 H5N1 流感病毒在小鼠中的致病性和神经毒力。
J Virol. 2012 Jun;86(12):6924-31. doi: 10.1128/JVI.07142-11. Epub 2012 Apr 11.
6
A multiplex RT-PCR assay for detection and differentiation of avian H3, H5, and H9 subtype influenza viruses and Newcastle disease viruses.一种用于检测和区分禽流感 H3、H5 和 H9 亚型流感病毒和新城疫病毒的多重 RT-PCR 检测方法。
J Virol Methods. 2012 May;181(2):164-9. doi: 10.1016/j.jviromet.2012.02.003. Epub 2012 Feb 21.
7
Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing.焦磷酸测序快速检测和亚型分析人甲型流感病毒及其重组。
PLoS One. 2011;6(8):e23400. doi: 10.1371/journal.pone.0023400. Epub 2011 Aug 19.
8
H5N1 influenza viruses: outbreaks and biological properties.H5N1 流感病毒：疫情爆发和生物学特性。
Cell Res. 2010 Jan;20(1):51-61. doi: 10.1038/cr.2009.124. Epub 2009 Nov 3.
9
Type-specific multiple sequencing primers: a novel strategy for reliable and rapid genotyping of human papillomaviruses by pyrosequencing technology.型特异性多重测序引物：一种通过焦磷酸测序技术对人乳头瘤病毒进行可靠且快速基因分型的新策略。
J Mol Diagn. 2005 May;7(2):198-205. doi: 10.1016/S1525-1578(10)60546-6.
10
Genetic variation analyses by Pyrosequencing.焦磷酸测序法进行的基因变异分析。
Mutat Res. 2005 Jun 3;573(1-2):96-102. doi: 10.1016/j.mrfmmm.2004.07.023.

焦磷酸测序法用于检测与H5N1禽流感病毒在哺乳动物中致病性增加相关的血凝素突变。

The use of pyrosequencing for detection of hemagglutinin mutations associated with increased pathogenicity of H5N1 avian influenza viruses in mammals.

作者信息

Wang Chenxi, Zhang Yongning, Bing Guoxia, Zhang Xuxiao, Wang Caixia, Wang Mingyang, Sun Yipeng, Wu Shaoqiang, Lin Xiangmei, Pu Juan, Liu Jinhua, Sun Honglei

机构信息

Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, and State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China (Chenxi Wang, X Zhang, M Wang, Y Sun, Pu, Liu, H Sun).

Chinese Academy of Inspection and Quarantine, Institute of Animal Quarantine, Chaoyang District, Beijing, China (Y Zhang, Caixia Wang, Wu, Lin).

出版信息

J Vet Diagn Invest. 2018 Jul;30(4):619-622. doi: 10.1177/1040638718769951. Epub 2018 Apr 10.

DOI:10.1177/1040638718769951
PMID:29633913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6505906/
Abstract

Hemagglutinin (HA) cleavage is critical for virulence of influenza viruses. The amino acid residue at the P6 position of the HA cleavage site (HACS) has been shown to be most variable and to have a direct correlation with the cleavage efficiency and pathogenicity of H5N1 avian influenza viruses (AIVs) in mammals. Among these amino acid variants, serine has been associated with the highest virulence in mammals, and its detection may serve as an indicator for H5N1 AIVs with high pathogenicity and potential public risk. We developed a rapid detection method based on reverse-transcription (RT)-PCR and pyrosequencing to detect a mutation at the HACS that is associated with increased pathogenicity of H5N1 AIVs in mammals. Herein, we provide a specific, sensitive, and reliable method for rapid detection of one of the virulence determinants associated with increased pathogenicity of H5N1 AIVs in mammals.

摘要

血凝素（HA）裂解对于流感病毒的毒力至关重要。HA裂解位点（HACS）的P6位置的氨基酸残基已被证明是最可变的，并且与H5N1禽流感病毒（AIVs）在哺乳动物中的裂解效率和致病性直接相关。在这些氨基酸变体中，丝氨酸与哺乳动物中最高的毒力相关，其检测可作为具有高致病性和潜在公共风险的H5N1 AIVs的指标。我们开发了一种基于逆转录（RT）-PCR和焦磷酸测序的快速检测方法，以检测与H5N1 AIVs在哺乳动物中致病性增加相关的HACS突变。在此，我们提供了一种特异性、灵敏且可靠的方法，用于快速检测与H5N1 AIVs在哺乳动物中致病性增加相关的毒力决定因素之一。