Lobo Carmen, Moreno-Ventas Xabier, Tapia-Paniagua Silvana, Rodríguez Covadonga, Moriñigo Miguel A, de La Banda Inés García
Spanish Institute of Oceanography, Oceanographic Center of Santander, Promontorio San Martín s/n. Apdo. 240, 39080, Santander, Spain,
Fish Physiol Biochem. 2014 Feb;40(1):295-309. doi: 10.1007/s10695-013-9844-0. Epub 2013 Aug 11.
Probiotic supplementation in fish aquaculture has significantly increased in the last decade due to its beneficial effect on fish performance. Probiotic use at early stages of fish development may contribute to better face metamorphosis and weaning stress. In the present work, we studied the influence of Shewanella putrefaciens Pdp11 supplementation on growth, body composition and gut microbiota in Senegalese sole (Solea senegalensis) during larval and weaning development. S. putrefaciens Pdp11 was incorporated using Artemia as live vector (2.5 × 10⁷ cfu mL⁻¹) and supplied to sole specimens in a co-feeding regime (10-86 DAH) by triplicate. Probiotic addition promoted early metamorphosis and a significantly higher growth in length at 24 DAH larvae. S. putrefaciens Pdp11 also modulated gut microbiota and significantly increased protein content and DHA/EPA ratios in sole fry (90 DAH). This nutritional enhancement is considered especially important after weaning, where significantly higher growth in length and weight was observed in probiotic fish. Moreover, a less heterogeneous fish size in length was detected since metamorphosis till the end of weaning, being of interest for sole aquaculture production. After weaning, fish showed significantly higher growth (length and weight) and less variable lengths in fish when supplemented with probiotics. Both the enhancement of nutritional condition and the decrease in size variability associated with probiotic addition are highly interesting for sole aquaculture production.
在过去十年中，由于益生菌对鱼类生长性能具有有益作用，其在鱼类养殖中的应用显著增加。在鱼类发育早期使用益生菌可能有助于更好地应对变态和断奶应激。在本研究中，我们探讨了添加腐败希瓦氏菌Pdp11对塞内加尔鳎（Solea senegalensis）幼体和断奶发育阶段生长、身体组成及肠道微生物群的影响。以卤虫作为活载体（2.5×10⁷ cfu mL⁻¹）引入腐败希瓦氏菌Pdp11，并以重复三次的方式在共投喂模式下（10 - 86日龄）投喂给鳎样本。添加益生菌促进了早期变态，并且在24日龄幼体时体长显著增加。腐败希瓦氏菌Pdp11还调节了肠道微生物群，并显著提高了鳎鱼苗（90日龄）的蛋白质含量和DHA/EPA比值。这种营养增强在断奶后尤为重要，在此阶段观察到添加益生菌的鱼体长和体重增长显著更高。此外，从变态期到断奶结束，检测到鱼的体长大小异质性降低，这对鳎鱼养殖生产具有重要意义。断奶后，添加益生菌的鱼体长和体重增长显著更高，且鱼体长度变异性更小。营养状况的改善以及与添加益生菌相关的大小变异性降低，对鳎鱼养殖生产都非常有意义。
