Ayad Magda M, Belal F, Hosney Mervet M, Elmansi Heba, Elsayed Nora
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Zagazig, Zagazig, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt.
J Chromatogr Sci. 2018 Jul 1;56(6):524-530. doi: 10.1093/chromsci/bmy027.
The proposed method describes a high performance liquid chromatographic method with fluoremetric detection for the determination of cisatracurium (CIS) and propofol (PRP) simultaneously, which are co-administered as a pre-operative injection mixture. The separation of the two drugs was achieved using monolithic column (100 mm and 4.6 mm internal diameter) and mixture of methanol and 0.1 M phosphate buffer in the ratio of 80:20 (v/v) at pH 4.5 as a mobile phase. The fluorescence detection was carried out at 230/324 nm. The procedure showed good linearity through the concentration ranges of 0.01-1.00 μg/mL and 0.1-3.0 μg/mL with limits of detection of 0.002, 0.030 μg/mL and limits of quantification of 0.006, 0.100 μg/mL for CIS and PRP, respectively. Simultaneous determination of CIS and PRP in spiked human plasma samples was additionally executed and the results were satisfactory precise and accurate.
所提出的方法描述了一种采用荧光检测的高效液相色谱法，用于同时测定顺式阿曲库铵（CIS）和丙泊酚（PRP），这两种药物作为术前注射混合剂共同给药。使用整体柱（内径100 mm和4.6 mm）以及甲醇和0.1 M磷酸盐缓冲液以80:20（v/v）的比例混合且pH值为4.5的混合液作为流动相，实现了两种药物的分离。荧光检测在230/324 nm波长下进行。该方法在0.01 - 1.00 μg/mL和0.1 - 3.0 μg/mL的浓度范围内显示出良好的线性，顺式阿曲库铵和丙泊酚的检测限分别为0.002、0.030 μg/mL，定量限分别为0.006、0.100 μg/mL。此外，还对加标人血浆样品中的顺式阿曲库铵和丙泊酚进行了同时测定，结果具有令人满意的精密度和准确性。
