Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University - Egypt, New Damietta, 34518, Egypt.
Luminescence. 2020 Mar;35(2):312-320. doi: 10.1002/bio.3729. Epub 2019 Nov 19.
Propofol and cisatracurium besylate have been simultaneously determined using a highly sensitive first derivative synchronous spectrofluorometric method. The method is based on measuring first derivative synchronous spectrofluorimetric amplitude at Δλ = 40 nm with a scanning rate of 600 nm/min. The different experimental parameters affecting the fluorescence intensity of the two drugs were carefully studied and optimized. The amplitude-concentration plots were rectilinear over the range 40.0-400.0 ng/mL and 20.0-280.0 ng/mL for propofol and cisatracurium, respectively with lower detection limits of 4.0 and 2.35 ng/mL and quantification limits of 12.1 and 7.1 ng/mL for propofol and cisatracurium, respectively. The proposed method was successfully applied for the determination of the two compounds in synthetic mixtures and in commercial ampoules. The high sensitivity attained using the proposed method allowed the simultaneous determination of both drugs in spiked plasma samples. The mean % recoveries in spiked human plasma (n = 3) were 96.53 ± 0.90 and 96.20 ± 1.64 for each of propofol and cisatracurium, respectively. The method was validated in compliance with International Council of Harmonization (ICH) Guidelines.
依托咪酯和顺苯磺酸阿曲库铵可以同时使用高灵敏度一阶导数同步荧光光谱法来进行测定。该方法基于以 600nm/min 的扫描速率在 Δλ=40nm 处测量一阶导数同步荧光光谱振幅。仔细研究并优化了影响两种药物荧光强度的不同实验参数。依托咪酯和顺苯磺酸阿曲库铵的幅度-浓度图在 40.0-400.0ng/mL 和 20.0-280.0ng/mL 范围内呈线性，检测限分别为 4.0 和 2.35ng/mL，定量限分别为 12.1 和 7.1ng/mL。该方法成功应用于合成混合物和商业安瓿中两种化合物的测定。所提出的方法具有较高的灵敏度，允许在加标血浆样品中同时测定这两种药物。在加标人血浆（n=3）中的平均回收率分别为 96.53±0.90%和 96.20±1.64%，分别为依托咪酯和顺苯磺酸阿曲库铵。该方法符合国际协调委员会（ICH）指南进行了验证。
