Department of Biomedical and Chemical Engineering, L.C. Smith College of Engineering and Computer Science, Syracuse University, Syracuse, New York, 13244.
Syracuse Biomaterials Institute, L.C. Smith College of Engineering and Computer Science, Syracuse University, Syracuse, New York, 13244.
J Biomed Mater Res B Appl Biomater. 2019 Jan;107(1):178-189. doi: 10.1002/jbm.b.34109. Epub 2018 Apr 10.
Osteosarcoma is a malignant bone cancer that occurs mostly in children and young adults. This study investigated the cytotoxicity of Mg and Mg-Ti microparticles to human osteosarcoma cells. Osteosarcoma cells were killed in a dosage-dependent manner when cells, with a cell seeding density of 30,000 cells/cm , were cultured with 0 to 2500 µg/mL of Mg or Mg-Ti in cell culture media for 24-72 h. Mg-Ti killed cells more effectively, where 1250 µg/mL of Mg-Ti killed cells completely by 24 h, while 2500 µg/mL of Mg killed nearly all cells, but not all. Killing due to particle corrosion occurred mostly during the first 24 h, and so the percent cell viability between 24 and 72 h showed not much variability. However, the measurement of live and dead cell numbers, over the timeframe of 24-72 h, showed more insight, such as cell recovery. If particle concentrations were low, the number of live cells increased after 24 h, indicating cell proliferation. If particle concentrations were high, the number of live cells either remained steady or decreased, indicating cell quiescence or continued killing, respectively. Increase in the number of dead cells also indicated killing, while plateau meant discontinued killing. In addition, repeated killing of recovered cells exhibited the same dose-dependent killing profile as the initial experiment, implying little development of cell resistance to treatment. These results, together, show that osteosarcoma cells are susceptible to killing by way of exposure to corroding particles, showing highly effective killing using the galvanic couple of Mg-Ti. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 178-189, 2019.
骨肉瘤是一种主要发生在儿童和青少年中的恶性骨癌。本研究探讨了镁和镁-钛微粒对人骨肉瘤细胞的细胞毒性。当细胞以 30000 个细胞/cm 的细胞接种密度在细胞培养液中与 0 至 2500 µg/mL 的镁或镁-钛孵育 24-72 小时时，细胞以剂量依赖性方式被杀死。镁-钛更有效地杀死细胞，其中 1250 µg/mL 的镁-钛在 24 小时内完全杀死细胞，而 2500 µg/mL 的镁杀死了几乎所有的细胞，但并非全部。由于颗粒腐蚀引起的杀伤主要发生在最初的 24 小时内，因此 24 至 72 小时之间的细胞活力百分比没有太大变化。然而，在 24-72 小时的时间范围内测量活细胞和死细胞的数量，可以更深入地了解细胞恢复情况。如果颗粒浓度较低，24 小时后活细胞数量增加，表明细胞增殖。如果颗粒浓度较高，活细胞数量要么保持稳定，要么减少，分别表明细胞静止或持续杀伤。死细胞数量的增加也表明杀伤，而平台则意味着停止杀伤。此外，对恢复的细胞进行重复杀伤显示出与初始实验相同的剂量依赖性杀伤谱，这意味着细胞对治疗的耐药性发展很少。这些结果表明，骨肉瘤细胞易受腐蚀颗粒暴露的杀伤，利用镁-钛的电偶对具有高度有效的杀伤作用。© 2018 Wiley Periodicals, Inc. J 生物医学材料研究杂志 B：应用生物材料，107B：178-189，2019。
