miR-182-5p 在肺鳞癌中的临床价值：一项结合 TCGA、GEO 数据和 RT-qPCR 验证的研究。
Clinical value of miR-182-5p in lung squamous cell carcinoma: a study combining data from TCGA, GEO, and RT-qPCR validation.
机构信息
Department of Medical Oncology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, Zhuang Autonomous Region, China.
Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, Zhuang Autonomous Region, China.
出版信息
World J Surg Oncol. 2018 Apr 10;16(1):76. doi: 10.1186/s12957-018-1378-6.
BACKGROUND
MiR-182-5p, as a member of miRNA family, can be detected in lung cancer and plays an important role in lung cancer. To explore the clinical value of miR-182-5p in lung squamous cell carcinoma (LUSC) and to unveil the molecular mechanism of LUSC.
METHODS
The clinical value of miR-182-5p in LUSC was investigated by collecting and calculating data from The Cancer Genome Atlas (TCGA) database, the Gene Expression Omnibus (GEO) database, and real-time quantitative polymerase chain reaction (RT-qPCR). Twelve prediction platforms were used to predict the target genes of miR-182-5p. Protein-protein interaction (PPI) networks and gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to explore the molecular mechanism of LUSC.
RESULTS
The expression of miR-182-5p was significantly over-expressed in LUSC than in non-cancerous tissues, as evidenced by various approaches, including the TCGA database, GEO microarrays, RT-qPCR, and a comprehensive meta-analysis of 501 LUSC cases and 148 non-cancerous cases. Furthermore, a total of 81 potential target genes were chosen from the union of predicted genes and the TCGA database. GO and KEGG analyses demonstrated that the target genes are involved in pathways related to biological processes. PPIs revealed the relationships between these genes, with EPAS1, PRKCE, NR3C1, and RHOB being located in the center of the PPI network.
CONCLUSIONS
MiR-182-5p upregulation greatly contributes to LUSC and may serve as a biomarker in LUSC.
背景
miR-182-5p 作为 miRNA 家族的一员，可在肺癌中检测到，并且在肺癌中发挥重要作用。探讨 miR-182-5p 在肺鳞癌（LUSC）中的临床价值，揭示 LUSC 的分子机制。
方法
通过收集和计算来自癌症基因组图谱（TCGA）数据库、基因表达综合数据库（GEO）和实时定量聚合酶链反应（RT-qPCR）的数据，研究 miR-182-5p 在 LUSC 中的临床价值。使用 12 个预测平台预测 miR-182-5p 的靶基因。采用蛋白质-蛋白质相互作用（PPI）网络和基因本体论（GO）、京都基因与基因组百科全书（KEGG）分析来探讨 LUSC 的分子机制。
结果
通过 TCGA 数据库、GEO 微阵列、RT-qPCR 和对 501 例 LUSC 病例和 148 例非癌性病例的综合荟萃分析，miR-182-5p 在 LUSC 中的表达明显高于非癌性组织。此外，从预测基因和 TCGA 数据库的联合中选择了总共 81 个潜在的靶基因。GO 和 KEGG 分析表明，这些靶基因参与与生物学过程相关的途径。PPI 揭示了这些基因之间的关系，EPAS1、PRKCE、NR3C1 和 RHOB 位于 PPI 网络的中心。
结论
miR-182-5p 的上调对 LUSC 有很大贡献，可能作为 LUSC 的生物标志物。
Zotero 插件