Department of Pharmacy, Faculty of Medicine and Pharmacy, University of Oradea, 29 Nicolae Jiga Street, 410028 Oradea, Romania.
Department of Physiology, "Iuliu Haţieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006 Cluj-Napoca, Romania.
Oxid Med Cell Longev. 2018 Feb 14;2018:3060525. doi: 10.1155/2018/3060525. eCollection 2018.
The antimicrobial activity of the L. extract and the mechanisms involved in the effects on endothelial vascular cells exposed to hyperosmotic stress were evaluated.
Antimicrobial activity was evaluated by disk diffusion method and minimum inhibitory concentration (MIC) determination, and oxidative stress, inflammation, and apoptosis, in pretreatment with L., caffeic acid, and cathechin, were quantified.
The results have shown that L. exhibited antibacterial effects only on pathogenic gram-positive cocci. The modulatory activity of L. on endothelial cells exposed to hypertonic medium was different and depended on the concentration used. Low concentrations of tested compounds exerted antioxidant effect and diminished the activity of caspase-8 and also increased IB expression while in high doses, L. was prooxidant, induced apoptosis, and decreased IL-6 secretion.
These experimental findings suggest that L. has antibacterial effects on gram-positive cocci and, administered in low dose, may be a new therapeutic approach for diseases associated with hypertonic conditions or oxidative stress and apoptosis.
评估了 L. 提取物的抗菌活性以及其对暴露于高渗应激的内皮血管细胞的影响所涉及的机制。
采用圆盘扩散法和最小抑菌浓度（MIC）测定法评估抗菌活性，并定量研究了 L.、咖啡酸和儿茶素预处理时的氧化应激、炎症和细胞凋亡。
结果表明，L. 仅对致病性革兰阳性球菌具有抗菌作用。L. 对高渗介质中内皮细胞的调节活性不同，取决于所用浓度。测试化合物的低浓度表现出抗氧化作用，降低了半胱天冬酶-8 的活性，同时增加了 IB 的表达，而在高剂量下，L. 是促氧化剂，诱导细胞凋亡，并减少了 IL-6 的分泌。
这些实验结果表明，L. 对革兰阳性球菌具有抗菌作用，并且以低剂量给药时，可能是与高渗条件或氧化应激和细胞凋亡相关的疾病的新治疗方法。
