微小RNA-22下调原癌基因ATP柠檬酸裂解酶以抑制乳腺癌的生长和转移。
MiR-22 down-regulates the proto-oncogene ATP citrate lyase to inhibit the growth and metastasis of breast cancer.
作者信息
Liu Huawen, Huang Xiaoping, Ye Tian
机构信息
Medical College of Soochow UniversityJiangsu, China.
Department of Oncology, Chongqing Three Gorges Central HospitalWanzhou, Chongqing, China.
出版信息
Am J Transl Res. 2018 Mar 15;10(3):659-669. eCollection 2018.
Abstract
Breast cancer, the most common malignancy in women worldwide, places a heavy economic burden and mental stress on families and society. Previous research showed that abnormal expression of miRNAs was closely related to the occurrence, metastasis, and angiogenesis of breast cancer. And in this study, the abnormal expression of miR-22 was detected by RT-PCR in the paired breast cancer tissues and adjacent non-tumor tissues. CCK-8 and wound healing assays were performed to evaluate the effects of the proto-oncogene ATP citrate lyase () on the growth and metastasis of breast cancer MCF-7 cells. The results showed that miR-22 inhibited the growth and metastasis of MCF-7 cells by down-regulating the expression of . In conclusion, this study elucidated the roles of miR-22 in regulation of breast cancer differentiation and migration, which provides a target for early diagnose and therapy of breast cancer.
摘要
乳腺癌是全球女性中最常见的恶性肿瘤，给家庭和社会带来了沉重的经济负担和精神压力。先前的研究表明，miRNAs的异常表达与乳腺癌的发生、转移和血管生成密切相关。在本研究中，通过RT-PCR检测配对的乳腺癌组织和相邻非肿瘤组织中miR-22的异常表达。进行CCK-8和伤口愈合试验以评估原癌基因ATP柠檬酸裂解酶（）对乳腺癌MCF-7细胞生长和转移的影响。结果表明，miR-22通过下调的表达来抑制MCF-7细胞的生长和转移。总之，本研究阐明了miR-22在调节乳腺癌分化和迁移中的作用，为乳腺癌的早期诊断和治疗提供了靶点。
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