Forouhar Vajargah Mohammad, Mohamadi Yalsuyi Ahmad, Hedayati Aliakbar, Faggio Caterina
Department of Fisheries, Faculty of Natural Resources, University of Guilan Sowmehsara, Iran.
Department of Aquaculture, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Microsc Res Tech. 2018 Jul;81(7):724-729. doi: 10.1002/jemt.23028. Epub 2018 Apr 10.
Different types of metal oxide nanoparticles (NPs) have been suggested for various applications such as water treatment and construction of agricultural pesticides; however, there are concerns about the potential toxicity of these compounds for the nontarget organism especially aquatic organisms. The aims of this study were assessing toxicity and histopathological effects of copper oxide NPs (NPs-CuO) on common carp (Cyprinus carpio) as a model organism. For this purpose 150 common carp with an average weight 7 ± 1 g were exposed to 0, 10, 20, 30, 40, 60, 80, 100, 150, and 200 mg/l of CuO-NPs (10 treatment with three replicates) for 96 hrs. After 24, 48, 72, and 96 hrs exposures, mortality rates recorded and gill samples were collected. Statistical analysis showed significant differences in carp survival between control and treatment groups (p < 0.05); regression between fish mortality rate and NPs-CuO concentration was also revealed (p < 0.01). The LC 96h of NPs-CuO for common carp was estimated as 124.9 mg/l in this study. Various tissue damages were observed in gill of treatments; such as, hypertrophy, hyperplasia, lamellar fusions, erythrocyte infiltration, epithelial lifting; also, there was significant correlation between intensity of tissue lesions and concentration of NPs-CuO (p < 0.01). The findings of the present study demonstrate that sublethal concentration of NPs-CuO can lead to serious tissue lesions. Whats more, concentrations above 30 ml/l of NPs-CuO can lead to some clinical signs; such as skin darkening and death with open mouth as well as definite fish death.
不同类型的金属氧化物纳米颗粒（NPs）已被建议用于各种应用，如水处理和农用杀虫剂的制造；然而，人们担心这些化合物对非目标生物尤其是水生生物的潜在毒性。本研究的目的是评估氧化铜纳米颗粒（NPs-CuO）对作为模式生物的鲤鱼（Cyprinus carpio）的毒性和组织病理学影响。为此，将150条平均体重为7±1克的鲤鱼暴露于0、10、20、30、40、60、80、100、150和200毫克/升的CuO-NPs中（10个处理组，每组3个重复），暴露96小时。在暴露24、48、72和96小时后，记录死亡率并采集鳃样本。统计分析表明，对照组和处理组之间鲤鱼的存活率存在显著差异（p<0.05）；还揭示了鱼类死亡率与NPs-CuO浓度之间的相关性（p<0.01）。本研究中，CuO-NPs对鲤鱼的96小时半数致死浓度（LC96h）估计为124.9毫克/升。在处理组的鳃中观察到了各种组织损伤；例如，肥大、增生、鳃小片融合、红细胞浸润、上皮隆起；此外，组织损伤的严重程度与NPs-CuO浓度之间存在显著相关性（p<0.01）。本研究结果表明，亚致死浓度的NPs-CuO可导致严重的组织损伤。此外，NPs-CuO浓度高于30毫克/升可导致一些临床症状；如皮肤变黑、张嘴死亡以及确定的鱼类死亡。
