Yalsuyi Ahmad Mohamadi, Vajargah Mohammad Forouhar, Hajimoradloo Abdolmajid, Galangash Mohsen Mohammadi, Prokić Marko D, Faggio Caterina
Department of Aquaculture, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 4913815739, Iran.
Department of Fisheries, Faculty of Natural Resources, University of Guilan, Sowmehsara 4199613776, Iran.
Vet Sci. 2021 Oct 5;8(10):218. doi: 10.3390/vetsci8100218.
Pesticides can induce changes in behavior and reduce the survival chance of aquatic organisms. In this study, the toxic effects of glyphosate suspension (Glyphosate Aria 41% SL, Tehran Iran) on behavior and tissues of common carp () were assessed. For this purpose, a 96 h LC of glyphosate suspension (68.788 mL·L) was used in the toxicity test. All individuals were divided into control and treatment groups with four replicates. Exposure operations were performed under two conditions: increasing concentration of suspension from 0 to 68.788 mL·L; then, decreasing to the first level. The swimming pattern was recorded by digital cameras during the test and tissue samples were collected at the end of the test. There were significant differences between the swimming pattern of treated individuals and control ones during both steps. The sublethal concentration of glyphosate led to hypertrophy, hyperplasia and hyperemia in the gill of fish. However, changes were obvious only after sampling. The exposed fish also displayed clinical signs such as darkening of the skin and increasing movement of the operculum. Moreover, glyphosate suspension affected swimming patterns of fish suggest that the swimming behavior test can indicate the potential toxicity of environmental pollutants and be used as a noninvasive, useful method for managing environmental changes and assessing fish health conditions by video monitoring.
农药可导致水生生物行为改变并降低其生存几率。在本研究中，评估了草甘膦悬浮剂（草甘膦阿瑞亚41% SL，伊朗德黑兰）对鲤鱼行为和组织的毒性作用。为此，在毒性试验中使用了草甘膦悬浮剂的96小时半数致死浓度（68.788 mL·L）。所有个体分为对照组和处理组，每组四个重复。暴露操作在两种条件下进行：悬浮剂浓度从0增加到68.788 mL·L；然后，降至初始水平。试验期间用数码相机记录游泳模式，并在试验结束时采集组织样本。在两个阶段，处理组个体和对照组个体的游泳模式均存在显著差异。草甘膦的亚致死浓度导致鱼鳃出现肥大、增生和充血。然而，这些变化仅在采样后才明显。暴露的鱼还表现出皮肤变黑和鳃盖活动增加等临床症状。此外，草甘膦悬浮剂对鱼游泳模式的影响表明，游泳行为试验可指示环境污染物的潜在毒性，并可作为一种通过视频监测管理环境变化和评估鱼类健康状况的非侵入性有用方法。
