Ji Min-Kyung, Oh Gyejeong, Kim Ji-Won, Park Sangwon, Yun Kwi-Dug, Bae Jung-Chan, Lim Hyun-Pil
J Nanosci Nanotechnol. 2017 Apr;17(4):2312-315. doi: 10.1166/jnn.2017.13328.
The aim of this study was to evaluate the antibacterial activity against Porphyromonas gingivalis and osteoblast viability of heat and plasma treatment of TiO2 nanotubes. Specimens were divided into four groups: the Ti (polished titanium), Nano (TiO2 nanotube), NH 300 (heat treated at 300 °C on TiO2 nanotube) and NH 400 (heat treated at 400 °C on TiO2 nanotube) groups. Antibacterial activity and osteoblast viability were evaluated in the four groups according to plasma treatment. Surface adhesion of Porphyromonas gingivalis was evaluated by crystal violet assay. Osteoblast viability was examined by XTT assay. Adhesion of Porphyromonas gingivalis was significantly decreased in the Ti group, Nano group and NH 300 group after plasma treatment (P < 0.05). Osteoblast viability was increased in the NH 400 group in comparison to the Ti group before plasma treatment (P < 0.05). Within the limitations of this study, plasma treatment was found to reduce the adhesion of P. gingivalis but had no influence on osteoblast activation.
本研究的目的是评估二氧化钛纳米管经热处理和等离子体处理后对牙龈卟啉单胞菌的抗菌活性以及对成骨细胞活力的影响。样本分为四组：钛组（抛光钛）、纳米组（二氧化钛纳米管）、NH 300组（二氧化钛纳米管在300℃下热处理）和NH 400组（二氧化钛纳米管在400℃下热处理）。根据等离子体处理情况评估四组的抗菌活性和成骨细胞活力。通过结晶紫测定法评估牙龈卟啉单胞菌的表面黏附情况。通过XTT测定法检测成骨细胞活力。等离子体处理后，钛组、纳米组和NH 300组中牙龈卟啉单胞菌的黏附显著降低（P < 0.05）。与等离子体处理前的钛组相比，NH 400组中的成骨细胞活力有所增加（P < 0.05）。在本研究的局限性范围内，发现等离子体处理可降低牙龈卟啉单胞菌的黏附，但对成骨细胞活化没有影响。
