Oh Soram, Cho Sung-In, Perinpanayagam Hiran, You Jinsu, Hong Seong-Hyeon, Yoo Yeon-Jee, Chang Seok Woo, Shon Won-Jun, Yoo Jun-Sang, Baek Seung-Ho, Kum Kee-Yeon
Department of Conservative Dentistry, Kyung Hee University Dental Hospital, Kyung Hee University School of Dentistry, 23 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Department of Conservative Dentistry, Dental Research Institute, Seoul National University Dental Hospital & Seoul Dental Hospital for Disabled, Seoul National University School of Dentistry, 101 Daehak-ro, Jongro-gu, Seoul 03080, Korea.
Materials (Basel). 2018 Apr 11;11(4):588. doi: 10.3390/ma11040588.
This study evaluated the sealing ability of gutta-percha (GP) with a calcium silicate-based sealer and a novel calcium zirconate containing calcium silicate cement (ZC). The root canals of the extracted premolars were prepared, which were then randomly allocated to three experimental groups (12 root canals per group) for obturation by continuous wave of condensation with the GP and AH 26 sealer (CW); obturation using a single GP with a calcium silicate-based EndoSequence BC sealer (SC); or obturation with ZC. The roots were inserted into sterile Eppendorf tubes, which were inoculated coronally with . The amount of endotoxin leakage into the apical reservoirs were measured using the Limulus Amebocyte Lysate (LAL) assay over 21 days, with comparisons made using one-way ANOVA and Scheffe's tests (α = 0.05). After 21 days, 75% of the canals that had been obturated by SC, 50% of those obturated by CW and 42% of those obturated by ZC showed endotoxin leakage. The amount of leakage was higher in the SC canals than in the CW ( = 0.031) or ZC ( = 0.03) canals, although there was no significant difference in the amount of leakage for CW and ZC ( > 0.05). X-ray diffraction revealed the presence of tricalcium silicate (Ca₃SiO₅) and calcium zirconate (CaZrO₃) in the synthesized ZC. Scanning electron microscopy revealed mineralized precipitates on the dentin of canals obturated by ZC. The novel calcium zirconate silicate cement appears to promote biomineralization and seal root canals at least as effectively as the conventional sealer.
本研究评估了牙胶（GP）与硅酸钙类封闭剂以及一种新型含硅酸钙的锆酸钙水泥（ZC）的封闭能力。制备了拔除前磨牙的根管，然后将其随机分为三个实验组（每组12个根管），分别采用连续波热压充填技术，用GP和AH 26封闭剂（CW）进行充填；使用单一GP和硅酸钙类EndoSequence BC封闭剂（SC）进行充填；或用ZC进行充填。将牙根插入无菌的艾本德管中，从冠方接种……采用鲎试剂法在21天内测量根尖储液器中内毒素渗漏的量，使用单因素方差分析和谢费检验进行比较（α = 0.05）。21天后，采用SC充填的根管中有75%出现内毒素渗漏，采用CW充填的为50%，采用ZC充填的为42%。SC组根管的渗漏量高于CW组（P = 0.031）或ZC组（P = 0.03），尽管CW组和ZC组的渗漏量无显著差异（P > 0.05）。X射线衍射显示合成的ZC中存在硅酸三钙（Ca₃SiO₅）和锆酸钙（CaZrO₃）。扫描电子显微镜显示，ZC充填的根管牙本质上有矿化沉淀。这种新型锆酸钙硅酸盐水泥似乎能促进生物矿化并封闭根管，其效果至少与传统封闭剂相当。
