Department of Histology and Embryology, Poznan University of Medical Sciences, Swiecickiego 6 Street, 60-781 Poznan, Poland.
Department of Anatomy and Histology, University of Zielona Gora, Zyty 28 Street, 65-046 Zielona Gora, Poland.
Int J Mol Sci. 2018 Apr 7;19(4):1111. doi: 10.3390/ijms19041111.
Compensatory adrenal growth evoked by unilateral adrenalectomy (hemiadrenalectomy) constitutes one of the most frequently studied in vivo models of adrenocortical enlargement. This type of growth has been quite well characterized for its morphological, biochemical, and morphometric parameters. However, the molecular basis of compensatory adrenal growth is poorly understood. Therefore, the aim of this study was to investigate the rat adrenal transcriptome profile during the time of two previously described adrenocortical proliferation waves at 24 and 72 h after unilateral adrenalectomy. Surgical removal of the left adrenal or a sham operation was accomplished via the classic dorsal approach. As expected, the weight of the remaining right adrenal glands collected at 24 and 72 h after hemiadrenalectomy increased significantly. The transcriptome profile was identified by means of Affymetrix Rat Gene 2.1 ST Array. The general profiles of differentially expressed genes were visualized as volcano plots and heatmaps. Detailed analyzes consisted of identifying significantly enriched gene ontological groups relevant to adrenal physiology, by means of DAVID and GOplot bioinformatics tools. The results of our studies showed that compensatory adrenal growth induced by unilateral adrenalectomy exerts a limited influence on the global transcriptome profile of the rat adrenal gland; nevertheless, it leads to significant changes in the expression of key genes regulating the circadian rhythm. Our results confirm also that regulation of compensatory adrenal growth is under complex and multifactorial control with a pivotal role of neural regulatory mechanisms and a supportive role of other components.
单侧肾上腺切除术（肾上腺切除术）引起的代偿性肾上腺生长是研究最多的体内肾上腺增大模型之一。这种类型的生长在形态、生化和形态计量学参数方面已经得到了很好的描述。然而，代偿性肾上腺生长的分子基础知之甚少。因此，本研究旨在研究大鼠肾上腺在单侧肾上腺切除后 24 和 72 小时两个先前描述的肾上腺增殖波期间的转录组谱。通过经典的背部方法进行左侧肾上腺切除术或假手术切除。正如预期的那样，在肾上腺切除术 24 和 72 小时后收集的剩余右侧肾上腺的重量显着增加。通过 Affymetrix Rat Gene 2.1 ST Array 鉴定转录组谱。通过火山图和热图可视化差异表达基因的一般图谱。详细分析包括通过 DAVID 和 GOplot 生物信息学工具确定与肾上腺生理学相关的显著富集基因本体组。我们的研究结果表明，单侧肾上腺切除术引起的代偿性肾上腺生长对大鼠肾上腺的全转录组谱的影响有限；然而，它导致调节昼夜节律的关键基因的表达发生显著变化。我们的研究结果还证实，代偿性肾上腺生长的调节受复杂的多因素控制，神经调节机制起关键作用，其他成分起辅助作用。
