噬菌体T4尾鞘基因的核苷酸序列及其产物的氨基酸序列。
Nucleotide sequence of the tail sheath gene of bacteriophage T4 and amino acid sequence of its product.
作者信息
Arisaka F, Nakako T, Takahashi H, Ishii S
机构信息
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
出版信息
J Virol. 1988 Apr;62(4):1186-93. doi: 10.1128/JVI.62.4.1186-1193.1988.
Abstract
The nucleotide sequence of gene 18 of bacteriophage T4 was determined by the Maxam-Gilbert method, partially aided by the dideoxy method. To confirm the deduced amino acid sequence of the tail sheath protein (gp18) that is encoded by gene 18, gp18 was extensively digested by trypsin or lysyl endopeptidase and subjected to reverse-phase high-performance liquid chromatography. Approximately 40 peptides, which cover 88% of the primary structure, were fractionated, the amino acid compositions were determined, and the corresponding sequences in DNA were identified. Furthermore, the amino acid sequences of 10 of the 40 peptides were determined by a gas phase protein sequencer, including N- and C-terminal sequences. Thus, the complete amino acid sequence of gp18, which consists of 658 amino acids with a molecular weight of 71,160, was determined.
摘要
通过马克萨姆-吉尔伯特法并部分借助双脱氧法测定了噬菌体T4基因18的核苷酸序列。为了确认由基因18编码的尾鞘蛋白（gp18）推导的氨基酸序列，用胰蛋白酶或赖氨酰内肽酶对gp18进行了充分消化，并进行反相高效液相色谱分析。分离出了约40个覆盖一级结构88%的肽段，测定了氨基酸组成，并鉴定了DNA中的相应序列。此外，用气相蛋白质测序仪测定了40个肽段中10个的氨基酸序列，包括N端和C端序列。由此确定了gp18由658个氨基酸组成、分子量为71160的完整氨基酸序列。
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