Akpınar Aysun, Karakan Nebı Cansın, Alpan Aysan Lektemur, Dogan Suat Serhan Altıntepe, Goze Fahrettin, Poyraz Omer
Srp Arh Celok Lek. 2016 May-Jun;144(5-6):273-9. doi: 10.2298/sarh1606273a.
Periodontitis is a chronic inflammatory and osteolytic disease. Vitamin B complex is a class of water-soluble vitamins that play important roles in cell metabolism.
The aim of this study was to evaluate the effects of riboflavin (RBF), nicotinamide (NA), and folic acid (FA) on alveolar bone loss in experimental periodontitis rat model.
Sixty-four male Wistar rats were randomly divided into the following eight groups: Control, Ligated, RBF50 (RBF, 50 mg/kg daily), NA50 (NA, 50 mg/kg daily), FA50 (FA, 50 mg/kg daily), RBF100 (RBF, 100 mg/kg daily), NA100 (NA, 100 mg/kg daily), and FA100 (FA, 100 mg/kg daily). Periodontitis was induced using silk ligature around the right first mandibular molar. After 11 days the rats were sacrificed. Mandible and serum samples were collected. Changes in alveolar bone levels were measured clinically, and periodontal tissues were examined histopathologically. Serum IL-1β (pg/ml) levels were analyzed by using ELISA.
Mean alveolar bone loss in the mandibular first molar tooth revealed to be significantly lower in RBF100 group than in the Control group. In the Ligated group, alveolar bone loss was significantly higher than in all other groups. The ratio of presence of inflammatory cell infiltration in the Ligated group was significantly higher than in the Control group. The differences in the serum IL-1β levels between the groups were not statistically significant. Osteoclasts that were observed in the Ligated group were significantly higher than those of the Control and FA100 groups. The osteoblastic activity in the Ligated group, RBF100, and NA100 groups were shown to be significantly higher than those in the Control group.
This study has demonstrated that systemic administration of RBF, NA, and FA in different dosages (50–100 mg/kg) reduced alveolar bone loss in periodontal disease in rats.
牙周炎是一种慢性炎症性溶骨疾病。复合维生素B是一类在细胞代谢中起重要作用的水溶性维生素。
本研究旨在评估核黄素（RBF）、烟酰胺（NA）和叶酸（FA）对实验性牙周炎大鼠模型牙槽骨丧失的影响。
64只雄性Wistar大鼠随机分为以下八组：对照组、结扎组、RBF50（RBF，每日50mg/kg）、NA50（NA，每日50mg/kg）、FA50（FA，每日50mg/kg）、RBF100（RBF，每日100mg/kg）、NA100（NA，每日100mg/kg）和FA100（FA，每日100mg/kg）。用丝线结扎右侧第一下颌磨牙诱导牙周炎。11天后处死大鼠。收集下颌骨和血清样本。临床测量牙槽骨水平的变化，并对牙周组织进行组织病理学检查。采用酶联免疫吸附测定法分析血清白细胞介素-1β（pg/ml）水平。
RBF100组下颌第一磨牙的平均牙槽骨丧失明显低于对照组。在结扎组中，牙槽骨丧失明显高于所有其他组。结扎组炎症细胞浸润存在率明显高于对照组。各组血清白细胞介素-1β水平差异无统计学意义。结扎组观察到的破骨细胞明显高于对照组和FA100组。结扎组、RBF100组和NA100组的成骨细胞活性明显高于对照组。
本研究表明，不同剂量（50-100mg/kg）的RBF、NA和FA全身给药可减少大鼠牙周疾病中的牙槽骨丧失。
