华支睾吸虫和麝猫后睾吸虫（吸虫纲，后睾科）的特异性和共同抗原
Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini(Opisthorchidae, Trematoda).
作者信息
Choi Min-Ho, Ryu Jin-Sook, Lee Mejeong, Li Shunyu, Chung Byung-Suk, Chai Jong-Yil, Sithithaworn Paiboon, Tesana Smarn, Hong Sung-Tae
机构信息
Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
出版信息
Korean J Parasitol. 2003 Sep;41(3):155-63. doi: 10.3347/kjp.2003.41.3.155.
Abstract
The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.
摘要
通过免疫印迹法分析了华支睾吸虫和麝猫后睾吸虫这两种人体肝吸虫的抗原特性及血清学反应。华支睾吸虫粗提物的抗原图谱包含8、26 - 28、34 - 37、43和70 kDa的主要蛋白质，而麝猫后睾吸虫的抗原图谱包含34 - 37、43、70和100 kDa的主要蛋白质。其中，华支睾吸虫中8、26 - 28和34 - 37 kDa的条带以及麝猫后睾吸虫中100 kDa的条带是各自排泄 - 分泌抗原的主要成分。8和26 - 28 kDa的条带对华支睾吸虫具有特异性，但麝猫后睾吸虫的100 kDa条带与华支睾吸虫病血清发生交叉反应，34 - 37、70和100 kDa的条带与其他蠕虫病血清发生交叉反应。免疫印迹反应的频率和强度与肝吸虫感染强度呈正相关。
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