Shahrivari-Baviloliaei Saba, Erdogan Orhan Ilkay, Abaci Kaplan Nurten, Konopacka Agnieszka, Waleron Krzysztof, Plenis Alina, Viapiana Agnieszka
Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, Poland.
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Türkiye.
Antioxidants (Basel). 2024 Aug 16;13(8):993. doi: 10.3390/antiox13080993.
Fisch. ex Bunge (syn. Bunge) is one of the notable medicinal and food plants. Therefore, the aim of this study was to calculate the phenolic composition and antioxidant, antimicrobial, as well as enzyme inhibitory [acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR)] activities with chemometric approaches of the hydromethanolic and water extracts of commercial samples. Ten individual phenolic compounds were determined using high-performance liquid chromatography (HPLC), and only quercetin was found at a level of above 80 µg/g DW in both extracts. Moreover, the highest antioxidant activity in DPPH, FRAP, ABTS, and CUPRAC assays was found in the sample containing the roots in loose form from USA. extracts displayed the inhibition zone diameters within the range from 10 to 22 mm antimicrobial activity against , while there were no inhibition zones in any extracts in case of . The extracts of showed an inhibition rate below 40% against TYR, and among tested extracts, only two samples were able to inhibit BChE with IC values of above 30 µg/mL. Correlation analysis showed a highly positive relationship between their phenolic composition and antioxidant activity. Concluding, the obtained results confirmed that commercial samples could be an important dietary source of natural antioxidants.
Fisch. ex Bunge（同义词Bunge）是著名的药用和食用植物之一。因此，本研究的目的是采用化学计量学方法计算市售样品的甲醇水提取物和水提取物中的酚类成分、抗氧化、抗菌以及酶抑制[乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和酪氨酸酶（TYR）]活性。使用高效液相色谱（HPLC）测定了10种单一酚类化合物，两种提取物中仅发现槲皮素含量高于80 μg/g干重。此外，在美国松散形式根的样品中，二苯基苦味酰基自由基（DPPH）、铁离子还原抗氧化能力（FRAP）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和铜离子还原抗氧化能力（CUPRAC）测定中抗氧化活性最高。提取物对[具体微生物名称未给出]显示出10至22毫米范围内的抑菌圈直径，而对[具体微生物名称未给出]的任何提取物中均无抑菌圈。[具体植物名称未给出]的提取物对酪氨酸酶的抑制率低于40%，在测试提取物中，只有两个样品能够抑制丁酰胆碱酯酶，其半数抑制浓度（IC）值高于30 μg/mL。相关性分析表明其酚类成分与抗氧化活性之间存在高度正相关。总之，所得结果证实市售样品可能是天然抗氧化剂的重要饮食来源。
