College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Department of Korean Medicine Rehabilitation, Kyung Hee University Medical Center, Seoul 02447, Republic of Korea.
Int J Mol Sci. 2022 Dec 2;23(23):15213. doi: 10.3390/ijms232315213.
Colorectal cancer cell (CRC) is the fourth most common cancer in the world. There are several chemotherapy drugs available for its treatment, though they have side effects. Cycloastragenol (CY) is a compound from Astragalus membranaceus (Fisch.) Bge known to be effective in aging, anti-inflammatory, anticancer, and anti-heart failure treatments. Although many studies have demonstrated the functions of CY in cancer cells, no studies have shown the effects of p53 in colon cancer cells. In this study, we found that CY reduces the viability of colon cancer cells in p53 wild-type cells compared to p53 null cells and HT29. Furthermore, CY induces apoptosis by p53 activation in a dose- and time-dependent manner. And it was confirmed that it affects the L5 gene related to p53. Additionally, CY enhanced p53 expression compared to when either doxorubicin or 5-FU was used alone. Altogether, our findings suggest that CY induces apoptosis via p53 activation and inhibits the proliferation of colon cancer cells. In addition, apoptosis occurs in colon cancer cells due to other factors. Moreover, CY is expected to have a combined effect when used together with existing treatments for colon cancer in the future.
结直肠癌（CRC）是全球第四大常见癌症。有几种化疗药物可用于治疗结直肠癌，但它们有副作用。环黄芪醇（CY）是一种从黄芪（膜荚黄芪）中提取的化合物，已知在抗衰老、抗炎、抗癌和抗心力衰竭治疗方面有效。尽管许多研究已经证明了 CY 在癌细胞中的功能，但没有研究表明 p53 在结肠癌细胞中的作用。在这项研究中，我们发现与 p53 缺失细胞和 HT29 相比，CY 在 p53 野生型细胞中降低了结肠癌细胞的活力。此外，CY 通过 p53 激活以剂量和时间依赖的方式诱导细胞凋亡。并且证实它会影响与 p53 相关的 L5 基因。此外，与单独使用阿霉素或 5-FU 相比，CY 增强了 p53 的表达。总之，我们的研究结果表明，CY 通过激活 p53 诱导细胞凋亡，并抑制结肠癌细胞的增殖。此外，由于其他因素，细胞凋亡发生在结肠癌细胞中。此外，CY 有望在未来与结肠癌的现有治疗方法联合使用时产生协同作用。
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