Noh L M, Khan M M, Melmon K L
Department of Medicine (Division of Clinical Pharmacology), Stanford University School of Medicine, CA 94305.
Dev Comp Immunol. 1988 Winter;12(1):177-87. doi: 10.1016/0145-305x(88)90035-3.
We have used the mixed leukocyte reaction assay (MLR) to compare the non-specific suppressor activity of fresh and short-term cultured peripheral blood rosetted lymphocytes from human umbilical cord blood. To a one way MLR between adult responders and irradiated adult stimulator cells, we added adult or cord blood mononuclear cells. Cord-blood derived mononuclear cells were more potent suppressors of the MLR than the adult peripheral blood mononuclear cells. When the fresh cord blood T cell fraction was separated from non-T cells by rosetting, the frequency and capacity of suppression fell, suggesting a role of non-T cells in this phenomenon. However, short-term culture (up to nine days) of cord blood derived T cells in the absence of mitogen resulted in a significant increase in their suppressive characteristics. Maternal cells were not used in any of these assays and the suppression was non specific. There was no HLA restriction to the response. The cord blood lymphocytes responded poorly to alloantigen when compared to the adult lymphocytes. In addition, there was a correlation between the allogeneic proliferative response and the suppressive capacity of the cord blood cells. Such a correlation was not found with the adult peripheral blood lymphocytes.
我们已使用混合淋巴细胞反应试验（MLR）来比较新鲜的和短期培养的人脐带血外周血玫瑰花结淋巴细胞的非特异性抑制活性。在成年应答者与经辐照的成年刺激细胞之间的单向MLR中，我们加入了成年或脐带血单个核细胞。源自脐带血的单个核细胞比成年外周血单个核细胞对MLR具有更强的抑制作用。当通过玫瑰花结法将新鲜脐带血T细胞组分与非T细胞分离时，抑制的频率和能力下降，这表明非T细胞在该现象中起作用。然而，在无有丝分裂原的情况下对源自脐带血的T细胞进行短期培养（长达九天）会导致其抑制特性显著增加。在任何这些试验中均未使用母体细胞，且抑制是非特异性的。对该反应不存在HLA限制。与成年淋巴细胞相比，脐带血淋巴细胞对同种异体抗原的反应较差。此外，同种异体增殖反应与脐带血细胞的抑制能力之间存在相关性。在成年外周血淋巴细胞中未发现这种相关性。
