Herrod H G, Valenski W R
J Clin Immunol. 1982 Oct;2(4):319-26. doi: 10.1007/BF00915074.
When compared to adult mononuclear cells, cord blood mononuclear cells demonstrated significantly decreased T-lymphocyte colony formation (1351 +/- 643 vs 592 +/- 862, P less than 0.01). This diminished colony-forming activity did not appear to be associated with impaired responsiveness to the stimulant phytohemagglutinin or with excessive suppressor-cell activity. Irradiation reduced the colony-forming capacity of cord blood mononuclear cells more than it did that of adult mononuclear cells. Depletion of adherent cells reduced cord blood mononuclear-cell colony-forming capacity by 40%, while similar treatment reduced adult colony formation by 10%. Lymphocyte proliferation in liquid culture of cord and adult cells was minimally affected by these procedures. The colony-forming capacity of cord blood could be enhanced by the addition of irradiated adult cells (284 +/- 72 vs 752 +/- 78, P less than 0.01). This enhancement was demonstrated to be due to a soluble factor produced by a population of irradiated adult cells depleted of the OKT8+ subpopulation of lymphocytes. These results indicate that the progenitor cells of T-lymphocyte colonies in cord blood have distinct biologic characteristics when compared to colony progenitors present in adult blood. This assay may prove to be useful in our efforts to understand the differentiation of T-cell function in man.
与成人单核细胞相比，脐血单核细胞的T淋巴细胞集落形成显著减少（1351±643对592±862，P<0.01）。这种集落形成活性的降低似乎与对刺激物植物血凝素的反应性受损或抑制细胞活性过高无关。辐照对脐血单核细胞集落形成能力的降低作用比对成人单核细胞的更大。去除贴壁细胞使脐血单核细胞集落形成能力降低40%，而类似处理使成人细胞集落形成降低10%。这些操作对脐血和成人细胞液体培养中的淋巴细胞增殖影响极小。添加辐照的成人细胞可增强脐血的集落形成能力（284±72对752±78，P<0.01）。已证明这种增强是由于一群去除了OKT8+淋巴细胞亚群的辐照成人细胞产生的一种可溶性因子所致。这些结果表明，与成人血液中的集落祖细胞相比，脐血中T淋巴细胞集落的祖细胞具有独特的生物学特性。该检测方法可能被证明对我们理解人类T细胞功能的分化有用。
