Department of Dermatovenereology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Wuhou District, 610041 Chengdu, Sichuan Province, China.
Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
J Mycol Med. 2018 Sep;28(3):492-501. doi: 10.1016/j.mycmed.2018.03.007. Epub 2018 Apr 10.
Skin and soft tissue infections (SSTIs) are very common in dermatology and the use of antimicrobial formulations are important in treating these diseases. With the increasing of drug-resistant strains, researchers need to find ways to enhance the effectiveness and/or reduce the drug resistance. Clioquinol was one of antiseptics that can inactivate microbes. It was lack of data of antimicrobial activity; meanwhile it was infrequently used in infection. In order to research the antimicrobial spectrum and activity of topical 3% clioquinol cream among common pathogenic microorganisms compared with other common topical pharmaceuticals, we used modified agar diffusion assay to judge drug susceptibility and compared with broth microdilution assay. Thirty strains of pathogenic fungi belonging to 14 species and 5 strains of pathogenic bacterium belonging to 4 species from clinic or standard strains were enrolled into the experiment. The inhibition zone around 3% clioquinol cream for all experiment isolates was observed. It could inhibit the growth of most fungal species with different strength, but the antibacterial activity was weak. For Candida tropicalis, Candida guilliermondii, Aspergillus terreus, Fusarium solani and Trichoderma harzianum, the inhibition zone was biggest among all the tested drugs. The antifungal activity for Dermatophytes and Candida albicans was moderate. Two assays had a degree of consistency. Based on results above, we identified the antifungal spectrum of 3% clioquinol cream was broad. The antimicrobial strength of 3% clioquinol cream depended on the species but it can act on most of the species.
皮肤和软组织感染（SSTIs）在皮肤科中非常常见，使用抗菌制剂对于治疗这些疾病非常重要。随着耐药菌株的增加，研究人员需要寻找方法来提高疗效和/或降低耐药性。氯己定是一种可以使微生物失活的防腐剂。它缺乏抗菌活性的数据，同时在感染中很少使用。为了研究与其他常用局部药物相比，局部 3%氯己定乳膏对常见致病微生物的抗菌谱和活性，我们使用改良琼脂扩散法判断药物敏感性，并与肉汤微量稀释法进行比较。从临床或标准菌株中选取了 30 株属于 14 种的致病真菌和 5 株属于 4 种的致病细菌进行实验。观察了 3%氯己定乳膏对所有实验分离株的周围抑制区。它可以抑制大多数真菌物种的生长，但抗菌活性较弱。对于热带假丝酵母、近平滑假丝酵母、土曲霉、茄病镰刀菌和哈茨木霉，在所有测试药物中，其抑制区最大。对皮肤癣菌和白色念珠菌的抗真菌活性中等。两种检测方法具有一定的一致性。基于以上结果，我们确定了 3%氯己定乳膏的抗真菌谱较广。3%氯己定乳膏的抗菌强度取决于菌种，但可以作用于大多数菌种。
