Ignatova Milena, Nachev Nasko, Spasova Mariya, Manolova Nevena, Rashkov Iliya, Naydenov Mladen
Laboratory of Bioactive Polymers, Institute of Polymers, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 103A, BG-1113 Sofia, Bulgaria.
Department of Microbiology, Agricultural University, BG-4000 Plovdiv, Bulgaria.
Polymers (Basel). 2022 Jan 18;14(3):367. doi: 10.3390/polym14030367.
Esca is a grapevine disease known for centuries which pertains to the group of so-called vine trunk diseases. and are the two main fungal pathogens associated with esca. Novel fibrous materials with antifungal properties based on poly(3-hydroxybutyrate) (PHB), polyvinylpyrrolidone (PVP) and 5-chloro-7-iodo-8-hydroxyquinoline (clioquinol, CQ) were developed. One-pot electrospinning ("" strategy) or electrospinning in conjunction with electrospraying ("" strategy) were applied to obtain the materials. The materials' morphology and their surface chemical composition were examined using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR). CQ incorporated in the bulk of the fibers or in PVP particles deposited on the fibers was in the amorphous phase, which was confirmed by differential scanning calorimetry (DSC) and X-ray diffraction analysis (XRD). The in vitro release of CQ depended on the composition of the electrospun materials and on their design. The performed microbiological screening revealed that, unlike the non-loaded mats, the fibrous mats loaded with CQ were effective in inhibiting the growth of the pathogenic and fungi. Therefore, the created materials are promising as active dressings for grapevine protection against esca.
葡萄枝干病害是一种已为人所知数百年的葡萄藤疾病，属于所谓的葡萄树干病害类别。[具体两种真菌病原体名称未给出]是与葡萄枝干病害相关的两种主要真菌病原体。基于聚（3-羟基丁酸酯）（PHB）、聚乙烯吡咯烷酮（PVP）和5-氯-7-碘-8-羟基喹啉（氯碘羟喹，CQ）开发了具有抗真菌特性的新型纤维材料。采用一锅法静电纺丝（“[具体策略名称未给出]”策略）或静电纺丝与电喷雾相结合（“[具体策略名称未给出]”策略）来制备这些材料。使用扫描电子显微镜（SEM）、X射线光电子能谱（XPS）和衰减全反射傅里叶变换红外光谱（ATR-FTIR）对材料的形态及其表面化学成分进行了研究。通过差示扫描量热法（DSC）和X射线衍射分析（XRD）证实，掺入纤维本体或沉积在纤维上的PVP颗粒中的CQ处于非晶相。CQ的体外释放取决于电纺材料的组成及其设计。所进行的微生物筛选表明，与未负载的垫子不同，负载CQ的纤维垫子能够有效抑制致病[具体两种真菌名称未给出]真菌的生长。因此，所制备的材料有望作为用于保护葡萄藤免受葡萄枝干病害侵害的活性敷料。
