Quadri Syed Raziuddin, Sathish Manda, Quach Ngoc Tung, Li Wen-Jun, Narsing Rao Manik Prabhu
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, 91431, Kingdom of Saudi Arabia.
Centro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca,, 3460000, Chile.
Curr Microbiol. 2025 Jan 22;82(3):100. doi: 10.1007/s00284-024-04059-z.
In the present study, the taxonomic position of Salinisphaera halophila (NZ_AYKF00000000) and Salinisphaera orenii (NZ_AYKH00000000) was re-evaluated. In addition, their metabolic potentials and mechanisms for mitigating stress conditions were determined. Comparisons of 16S rRNA gene sequences, analysis of the phylogenetic tree, phylogenomic tree, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) values were conducted. The 16S rRNA gene sequence similarity between Salinisphaera halophila YIM 95161 and Salinisphaera orenii MK-B5 was 100%. In phylogenetic and phylogenomic trees, Salinisphaera halophila YIM 95161 and Salinisphaera orenii MK-B5clustered together. Both species encode genes for glycolysis, citrate cycle, pentose phosphate pathway, Entner-Doudoroff pathway, nitrate assimilation, and assimilatory sulfate reduction. They employ salt-in and salt-out strategies to mitigate salt stress. The ANI and dDDH values between Salinisphaera halophila YIM 95161and Salinisphaera orenii MK-B5were 96.6 and 72.1%, respectively, above the cut-off (95-96% for ANI and 70% for dDDH) for species delineation. Based on the above results, we propose to reclassify Salinisphaera halophila Zhang et al. 2012 as a later heterotypic synonym of Salinisphaera orenii Park et al. 2012.
在本研究中，对嗜盐盐球菌（NZ_AYKF00000000）和奥伦盐球菌（NZ_AYKH00000000）的分类地位进行了重新评估。此外，还确定了它们的代谢潜力和缓解应激条件的机制。进行了16S rRNA基因序列比较、系统发育树、系统基因组树分析、平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分析。嗜盐盐球菌YIM 95161与奥伦盐球菌MK-B5之间的16S rRNA基因序列相似性为100%。在系统发育树和系统基因组树中，嗜盐盐球菌YIM 95161和奥伦盐球菌MK-B5聚集在一起。这两个物种都编码参与糖酵解、柠檬酸循环、磷酸戊糖途径、Entner-Doudoroff途径、硝酸盐同化和同化性硫酸盐还原的基因。它们采用盐进和盐出策略来缓解盐胁迫。嗜盐盐球菌YIM 95161和奥伦盐球菌MK-B5之间的ANI和dDDH值分别为96.6%和72.1%，高于物种划分的阈值（ANI为95 - 96%，dDDH为70%）。基于上述结果，我们建议将2012年张等人描述的嗜盐盐球菌重新分类为2012年帕克等人描述的奥伦盐球菌的晚出异名。
