Beijing Key Laboratory of Work Safety Intelligent Monitoring, School of Electronic Engineering, Beijing University of Posts and Telecommunications, Haidian District, Beijing, 100876, People's Republic of China.
Methods Appl Fluoresc. 2018 Apr 27;6(3):035008. doi: 10.1088/2050-6120/aabe03.
Ratiometric fluorescent nanoparticles (NPs) under two-photon excitation are successfully developed for sensing dissolved oxygen. The NPs comprise the oxygen probe Pt(II)-porphyrins (PtTFPP) and fluorescent organic semiconducting polymer (PFO). PFO polymer acts as both a two-photon antenna and a reference dye, while PtTFPP absorbs the photonic energy transferred by the PFO under two-photon excitation at 740 nm to sense oxygen. The red fluorescence of PtTFPP is sensitive to oxygen with a quenching response of 88% from nitrogen saturation to oxygen saturation, and PFO gives oxygen-insensitive referenced blue fluorescence. The fluorescence quenching of the NPs against oxygen at two-photon excitation follows a linear Stern-Volmer behavior. The nanosensors exhibit low cytotoxic effects as well as effortless cellular uptake. When incorporated into cells, the ratio of the signals increases up to about 500% from oxygen-saturated to oxygen-free environment.
比率型双光子激发荧光纳米粒子（NPs）成功开发用于感测溶解氧。 NPs 由氧探针 Pt(II)-卟啉（PtTFPP）和荧光有机半导体聚合物（PFO）组成。 PFO 聚合物既是双光子天线又是参比染料，而 PtTFPP 在 740nm 处吸收 PFO 转移的光子能量，通过双光子激发来感测氧。 PtTFPP 的红色荧光对氧敏感，从氮气饱和到氧气饱和的猝灭响应为 88%，而 PFO 给出对氧不敏感的参比蓝色荧光。 NPs 在双光子激发下对氧的荧光猝灭遵循线性 Stern-Volmer 行为。 纳米传感器表现出低细胞毒性和易于细胞摄取。 当掺入细胞中时，信号的比率从氧饱和到无氧环境增加高达约 500%。
