Liang Qiankun, Yan Yuan, Mao Lanfang, Du Xiaojuan, Liang Jingjing, Liu Junhong, Wang Longde, Li Hongfang
Department of Physiology, College of Basic Medicine, Lanzhou University, Lanzhou, China.
Department of Gastroenterology, Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, China.
Saudi J Gastroenterol. 2018 Jul-Aug;24(4):228-235. doi: 10.4103/sjg.SJG_505_17.
BACKGROUND/AIM: The purpose of this study was to establish a modified rat model with functional dyspepsia (FD) and analyze the changes in gastrointestinal motility and brain-gut peptide levels in serum and brain-gut axis.
Male Wistar rats were divided into control group (Con) and FD model group. FD model was established by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue until gastrointestinal motility disorder appeared, and then levels of motilin, leptin, cholecystokinin (CCK), and vasoactive intestinal peptide (VIP) were detected in serum by enzyme linked immunosorbent assay and in duodenum, antrum, and hypothalamus by immunohistochemistry, reverse transcriptase-polymerase chain reaction, and Western blot.
The results showed rates of intestinal propulsion and gastric emptying slowed down markedly compared to Con (P < 0.05), the gastrointestinal electric activity attenuated, and migrating motor complex (MMC) interrupted in the model group. The levels of leptin and VIP markedly increased, but motilin decreased as compared to the Con (P < 0.05) in serum and in the above tissues. It is interesting that the level of CCK decreased in the antrum and duodenum but increased in the hypothalamus as compared to Con (P < 0.05).
The modified rat model meets the diagnostic criteria of FD and can be used as a method for studying FD in animals.
背景/目的：本研究旨在建立一种改良的功能性消化不良（FD）大鼠模型，并分析胃肠动力以及血清和脑-肠轴中脑肠肽水平的变化。
将雄性Wistar大鼠分为对照组（Con）和FD模型组。通过对半饥饿大鼠进行夹尾、激怒及强迫运动疲劳刺激，直至出现胃肠动力障碍来建立FD模型，然后采用酶联免疫吸附测定法检测血清中胃动素、瘦素、胆囊收缩素（CCK）和血管活性肠肽（VIP）的水平，并通过免疫组织化学、逆转录-聚合酶链反应和蛋白质印迹法检测十二指肠、胃窦和下丘脑组织中的上述指标。
结果显示，与对照组相比，模型组的肠推进率和胃排空率显著减慢（P<0.05），胃肠电活动减弱，移行性运动复合波（MMC）中断。血清及上述组织中，与对照组相比，瘦素和VIP水平显著升高，而胃动素水平降低（P<0.05）。有趣的是，与对照组相比，胃窦和十二指肠中CCK水平降低，而下丘脑中CCK水平升高（P<0.05）。
改良的大鼠模型符合FD的诊断标准，可作为动物研究FD的一种方法。
