Liang Qiankun, Li Yunxia, Yan Yuan, Liang Jingjing, Mao Lanfang, Liu Junhong, Wang Longde, Du Xiaojuan, Li Hongfang
Department of Physiology, College of Basic Medicine, Lanzhou University, Lanzhou 730000, China.
Department of Gastroenterology, Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730020, China.
J Tradit Chin Med. 2018 Feb;38(1):43-53.
To investigate the mechanism of Pingwei capsules (PWC) in improving gastrointestinal motility in rats with functional dyspepsia (FD).
We established an FD model by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue. The FD model group was further divided into five groups according to the treatment received: normal saline, domperidone, low-dose PWC, mid-dose PWC, or high- dose PWC. The effect of PWC on FD rat was evaluated by measuring gastrointestinal motility. Changes of leptin and cholecystokinin (CCK) were detected through enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and immunohistochemistry.
PWC significantly increased gastrointestinal motility in FD rats. Furthermore, PWC significantly increased CCK mRNA and protein concentrations in the duodenum and antrum, decreased leptin protein concentrations in the duodenum, antrum, and hypothalamus, and decreased CCK protein concentration in the hypothalamus.
PWC improve gastrointestinal motor function in FD rats by decreasing the leptin concentration in serum and the brain-gut axis, and by increasing the CCK concentration in gastrointestinal tissue. Our findings help to elucidate the mechanism of FD and provide further insight into the pharmacokinetics of PWC.
探讨平胃胶囊（PWC）改善功能性消化不良（FD）大鼠胃肠动力的机制。
通过尾端电击、激怒及强迫运动疲劳刺激半饥饿大鼠建立FD模型。FD模型组根据接受的治疗进一步分为五组：生理盐水组、多潘立酮组、低剂量PWC组、中剂量PWC组或高剂量PWC组。通过测量胃肠动力评估PWC对FD大鼠的作用。采用酶联免疫吸附测定、逆转录-聚合酶链反应和免疫组织化学检测瘦素和胆囊收缩素（CCK）的变化。
PWC显著增加FD大鼠的胃肠动力。此外，PWC显著增加十二指肠和胃窦中CCK的mRNA和蛋白浓度，降低十二指肠、胃窦和下丘脑的瘦素蛋白浓度，并降低下丘脑的CCK蛋白浓度。
PWC通过降低血清和脑-肠轴中的瘦素浓度以及增加胃肠组织中的CCK浓度来改善FD大鼠的胃肠运动功能。我们的研究结果有助于阐明FD的机制，并为PWC的药代动力学提供进一步的见解。
