Bouacha Mabrouka, Ayed Hayette, Grara Nedjoud
Laboratory of Biochemistry and Microbiology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar,23000 Annaba, Algeria.
Department of Biology, Faculty of Biology, University 8 May 1945, 24000 Guelma, Algeria.
Sci Pharm. 2018 Apr 13;86(2):14. doi: 10.3390/scipharm86020014.
Medicinal benefits of honey bee have been recognized in the medical community since ancient times as a remedy for many diseases and infections. This study aimed to investigate the susceptibility of 11 multidrug-resistant bacterial strains, isolated from urinary tract infections of pregnant women, to six honey samples collected from different localities in the east of Algeria. The evaluation of the antibacterial activity was performed by the well method followed by the broth dilution method using two-fold dilutions of each honey sample ranging from 2.5 to 80% (w/v). The results obtained in this study revealed that all tested honeys exhibited potent antibacterial activity against the tested strains. The diameters of inhibition ranged from 19.67 to 53.33 mm, with minimum inhibitory concentrations (MICs) ranging from 2.5 to 40% (w/v) and minimum bactericidal concentration (MBCs) varied between 2.5 and 80% (w/v). Gram-positive bacteria were found to be more susceptible than Gram-negative bacteria with diameters ranging from 43.33 to 53.33 mm; MIC and MBC values ranged from 2.5 to 5% (w/v). The strain was found to be less susceptible than other strains with inhibitory diameters ranging from 19.67 to 27.33 mm; MICs ranged from 20 to 40% and MBCs ranged from 20 to 80% (). This contribution has provided a broad overview of the antibacterial activity of Algerian honey and shown that honey bee has great potential for therapeutic use as an alternative therapy for urinary tract infection treatment which is safe and efficient during pregnancy.
自古以来，医学界就认识到蜜蜂的药用价值，它可作为多种疾病和感染的治疗方法。本研究旨在调查从孕妇尿路感染中分离出的11种多重耐药细菌菌株对从阿尔及利亚东部不同地区采集的6份蜂蜜样本的敏感性。抗菌活性评估采用打孔法，随后采用肉汤稀释法，对每个蜂蜜样本进行2.5%至80%（w/v）的两倍稀释。本研究获得的结果表明，所有测试蜂蜜对测试菌株均表现出强大的抗菌活性。抑菌圈直径在19.67至53.33毫米之间，最低抑菌浓度（MIC）在2.5%至40%（w/v）之间，最低杀菌浓度（MBC）在2.5%至80%（w/v）之间变化。发现革兰氏阳性菌比革兰氏阴性菌更敏感，抑菌圈直径在43.33至53.33毫米之间；MIC和MBC值在2.5%至5%（w/v）之间。该菌株比其他菌株更不敏感，抑菌直径在19.67至27.33毫米之间；MIC在20%至40%之间，MBC在20%至80%（）之间。本研究全面概述了阿尔及利亚蜂蜜的抗菌活性，并表明蜜蜂作为尿路感染治疗的替代疗法具有巨大的治疗潜力，在孕期安全有效。
