Kim SeGun, Hong InPyo, Woo SoonOk, Jang HyeRi, Pak SokCheon, Han SangMi
Department of Agricultural Biology, National Institute of Agricultural Science, Wanju, Korea.
School of Biomedical Sciences, Charles Sturt University, Bathurst, NSW, Australia.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S170-S173. doi: 10.4103/0973-1296.210166. Epub 2017 Jul 11.
() is linked to the development of the majority of peptic ulcers and some types of gastric cancers, and its antibiotic resistance is currently found worldwide.
This study is aimed at evaluating the anti- activity of Korean acacia honey and isolating the related active components using organic solvents.
The crude acacia honey was extracted with -hexane, dichloromethane, ethyl acetate (EtOAc), and -butanol. The EtOAc extract was subjected to octadecyl-silica chromatography. The extracts and fractions were then examined for anti- activity using the agar well diffusion method. The antimicrobial activity of abscisic acid against was investigated by determining the minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), and by performing a time-kill assay.
Abscisic acid related to the botanical origins of acacia honey from Korea has been analyzed using ultra-performance liquid chromatography. The MICs and MBCs of abscisic acid were 2.7 ± 1.3 and 6.9 ± 1.9 μg/mL, respectively. The bactericidal activity of abscisic acid (at 10.8 μg/mL corresponding to 4 × MIC) killed the organism within 36-72 h. These results suggest that abscisic acid isolated from Korean acacia honey has antibacterial activity against .
Abscisic acid isolated from Korean acacia honey can be therapeutic and may be further exploited as a potential lead candidate for the development of treatments for -induced infections.
The crude acacia honey was extracted with -hexane, dichloromethane, EtOAc, and -butanolThe EtOAc extract yielded eight fractions and four subfractions were subsequently obtained chromatographicallyAbscisic acid was isolated from one subfractionAll the solvent extracts and fractions showed antibacterial activity against Abscisic acid exhibited antibacterial activity against . MeOH: Methanol; EtOAc: Ethyl acetate; TSB: Trypticase soy broth; MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; CFU: Colony-forming units; UPLC: Ultra-performance liquid chromatography; DAD: Diode array detector; UV: Ultraviolet; ODS: Octadecyl-silica; MS: Mass spectrometry; SE: Standard error.
（）与大多数消化性溃疡和某些类型胃癌的发生有关，并且其抗生素耐药性目前在全球范围内均有发现。
本研究旨在评估韩国相思树蜂蜜的抗（）活性，并使用有机溶剂分离相关活性成分。
粗相思树蜂蜜用正己烷、二氯甲烷、乙酸乙酯（EtOAc）和正丁醇进行萃取。乙酸乙酯提取物进行十八烷基硅胶柱色谱分离。然后使用琼脂孔扩散法检测提取物和馏分的抗（）活性。通过测定最低抑菌浓度（MICs）、最低杀菌浓度（MBCs）并进行时间 - 杀菌试验，研究脱落酸对（）的抗菌活性。
已使用超高效液相色谱法分析了与韩国相思树蜂蜜植物来源相关的脱落酸。脱落酸的MICs和MBCs分别为2.7±1.3和6.9±1.9μg/mL。脱落酸（在10.8μg/mL，相当于4×MIC）的杀菌活性在36 - 72小时内杀灭了该菌。这些结果表明，从韩国相思树蜂蜜中分离出的脱落酸对（）具有抗菌活性。
从韩国相思树蜂蜜中分离出的脱落酸具有治疗作用，并且可能作为治疗由（）引起感染的潜在先导候选物被进一步开发利用。
粗相思树蜂蜜用正己烷、二氯甲烷、乙酸乙酯和正丁醇进行萃取。乙酸乙酯提取物产生了八个馏分，随后通过色谱法获得了四个亚馏分。从一个亚馏分中分离出了脱落酸。所有溶剂提取物和馏分均对（）显示出抗菌活性。脱落酸对（）表现出抗菌活性。 甲醇：MeOH；乙酸乙酯：EtOAc；胰蛋白胨大豆肉汤：TSB；最低抑菌浓度：MIC；最低杀菌浓度：MBC；菌落形成单位：CFU；超高效液相色谱：UPLC；二极管阵列检测器：DAD；紫外线：UV；十八烷基硅胶：ODS；质谱：MS；标准误差：SE。
