Gastrointestinal Colorectal and Anal surgery, China-Japan Union Hospital of Jilin University, No. 126 Xiantai Street, Changchun, Jilin, 130033, China.
Department of Neurology, The First Hospital of Jilin University, Changchun, Jilin, 130021, China.
Biol Res. 2018 Apr 13;51(1):10. doi: 10.1186/s40659-018-0158-7.
We aimed to explore the potential molecular mechanism and independent prognostic genes for colon cancer (CC).
Microarray datasets GSE17536 and GSE39582 were downloaded from Gene Expression Omnibus. Meanwhile, the whole CC-related dataset were downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed mRNA (DEMs) were identified between cancer tissue samples and para-carcinoma tissue samples in TCGA dataset, followed by the KEGG pathway and GO function analyses. Furthermore, the clinical prognostic analysis including overall survival (OS) and disease-free survival (DFS) were performed in all three datasets.
A total of 633 up- and 321 down-regulated mRNAs were revealed in TCGA dataset. The up-regulated mRNAs were mainly assembled in functions including extracellular matrix and pathways including Wnt signaling. The down-regulated mRNAs were mainly assembled in functions like Digestion and pathways like Drug metabolism. Furthermore, up-regulation of UL16-binding protein 2 (ULBP2) was associated with OS in CC patients. A total of 12 DEMs including Surfactant Associated 2 (SFTA2) were potential DFS prognostic genes in CC patients. Meanwhile, the GRP and Transmembrane Protein 37 (TMEM37) were two outstanding independent DFS prognostic genes in CC.
ULBP2 might be a potential novel OS prognostic biomarker in CC, while GRP and TMEM37 could be served as the independent DFS prognostic genes in CC. Furthermore, functions including extracellular matrix and digestion, as well as pathways including Wnt signaling and drug metabolism might play important roles in the process of CC.
我们旨在探索结肠癌（CC）的潜在分子机制和独立的预后基因。
从基因表达综合数据库中下载微阵列数据集 GSE17536 和 GSE39582。同时，从癌症基因组图谱（TCGA）数据库中下载整个 CC 相关数据集。在 TCGA 数据集中，鉴定癌症组织样本与癌旁组织样本之间的差异表达 mRNA（DEMs），然后进行 KEGG 通路和 GO 功能分析。此外，在所有三个数据集中进行包括总生存期（OS）和无病生存期（DFS）在内的临床预后分析。
TCGA 数据集中共发现 633 个上调和 321 个下调的 mRNAs。上调的 mRNAs 主要组装在包括细胞外基质在内的功能中，途径包括 Wnt 信号。下调的 mRNAs 主要组装在消化等功能中，途径包括药物代谢。此外，UL16 结合蛋白 2（ULBP2）的上调与 CC 患者的 OS 相关。在 CC 患者中，SFTA2 等共 12 个 DEMs 是潜在的 DFS 预后基因。同时，GRP 和跨膜蛋白 37（TMEM37）是 CC 两个杰出的独立 DFS 预后基因。
ULBP2 可能是 CC 中潜在的新 OS 预后生物标志物，而 GRP 和 TMEM37 可作为 CC 的独立 DFS 预后基因。此外，包括细胞外基质和消化在内的功能，以及包括 Wnt 信号和药物代谢在内的途径，可能在 CC 过程中发挥重要作用。
