Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India.
Appl Biochem Biotechnol. 2018 Oct;186(2):476-495. doi: 10.1007/s12010-018-2755-x. Epub 2018 Apr 14.
Resveratrol is extensively being used as a therapeutic moiety, as well as a pharmacophore for development of new drugs due to its multifarious beneficial effects. The objective of the present study was to isolate and screen the resveratrol-producing endophytic fungi from different varieties of Vitis vinifera. A total of 53 endophytic fungi belonging to different fungal genera were isolated from the stem and leaf tissues of Vitis vinifera (merlot, wild, pinot noir, Shiraz, muscat) from different grape-producing locations of India. Only 29 endophytic fungal isolates exhibited a positive test for phenolics by phytochemical methods. The resveratrol obtained after ethyl acetate extraction was confirmed using standard molecule on thin layer chromatography (TLC) with a retention factor (R) of 0.69. The purified and standard resveratrol were visualized under UV light as a violet-colored spot. In HPLC analysis of the ethyl acetate extract of culture broth of 11 endophytic isolates, the highest resveratrol content was found in #12VVLPM (89.1 μg/ml) followed by #18VVLPM (37.3 μg/ml) and 193VVSTPM (25.2 μg/ml) exhibiting a retention time of 3.36 min which corresponded to the standard resveratrol. The resveratrol-producing isolates belong to seven genera viz. Aspergillus, Botryosphaeria, Penicillium, Fusarium, Alternaria, Arcopilus, and Lasiodiplodia, and using morphological and molecular methods, #12VVLPM was identified as Arcopilus aureus.
白藜芦醇因其多种有益作用而被广泛用作治疗药物，也是开发新药的药效团。本研究的目的是从不同品种的葡萄(Vitis vinifera)中分离和筛选产生白藜芦醇的内生真菌。从印度不同葡萄种植区的葡萄(Vitis vinifera)茎和叶组织中分离出 53 种属于不同真菌属的内生真菌，其中包括梅洛、野生、黑比诺、设拉子、麝香等不同品种。只有 29 种内生真菌分离物通过植物化学方法对酚类物质呈阳性反应。用薄层色谱法（TLC）用标准分子确认从乙酸乙酯提取物中得到的白藜芦醇，保留因子（R）为 0.69。纯化和标准白藜芦醇在紫外光下显示为紫罗兰色斑点。在 11 种内生菌培养液的乙酸乙酯提取物的 HPLC 分析中，#12VVLPM 的白藜芦醇含量最高（89.1μg/ml），其次是#18VVLPM（37.3μg/ml）和 193VVSTPM（25.2μg/ml），保留时间为 3.36min，与标准白藜芦醇相对应。产生白藜芦醇的分离物属于七个属，即曲霉属、丛赤壳属、青霉属、镰刀菌属、交链孢属、Arcopilus 和双极属，通过形态学和分子方法，#12VVLPM 被鉴定为金黄丛赤壳。
