Benaki Dimitra, Mikros Emmanuel
Department of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Athens, Greece.
Methods Mol Biol. 2018;1738:117-131. doi: 10.1007/978-1-4939-7643-0_8.
Metabolomic studies offer a wealth of information on cells, tissues, and biofluids. The phenotype representation through the metabolic profiling is a valuable tool for direct diagnosis, therapeutic strategies, and system's biology studies. Nuclear magnetic resonance (NMR) spectroscopy provides a nondestructive and extremely reproducible method allowing simultaneous detection of a large number of known and unknown chemical substances.Sample collection and preparation and experimental conditions are critical for the reliability of the subsequent analysis. The pre-analytical phase is decisive as it could generate biased spectral data misleading the following analysis. The formulation of standard operating procedures is thus of crucial importance in order to access meaningful samples and results. In this protocol, we provide standardized operations and routine procedures from sample preparation to determine the measurement details for the acquisition of NMR spectra highlighting major methodological issues.
代谢组学研究提供了有关细胞、组织和生物流体的丰富信息。通过代谢谱进行表型表征是直接诊断、治疗策略和系统生物学研究的宝贵工具。核磁共振（NMR）光谱提供了一种无损且极具可重复性的方法，可同时检测大量已知和未知的化学物质。样品采集、制备以及实验条件对于后续分析的可靠性至关重要。分析前阶段具有决定性作用，因为它可能会产生有偏差的光谱数据，从而误导后续分析。因此，制定标准操作程序对于获取有意义的样本和结果至关重要。在本方案中，我们提供了从样品制备到确定用于获取NMR光谱的测量细节的标准化操作和常规程序，突出了主要的方法学问题。
