College of Materials and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China.
College of Materials and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China.
Int J Biol Macromol. 2018 Aug;115:811-819. doi: 10.1016/j.ijbiomac.2018.04.054. Epub 2018 Apr 12.
We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro. The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL. Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/mL, exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration. A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established. This result suggests that CCP can be used as hypoglycemic medicine or health food.
我们从油茶籽饼（CCP）中分离并纯化了多糖，并研究了其在体外的降血糖活性。CCP 的分子量为 4736 Da，单糖摩尔组成比为：木糖：葡萄糖醛酸：氨基半乳糖：甘露糖= 10.9：4.4：2.6：1.8。CCP 含有结合蛋白，属于酸性多糖，可在 0.125-0.500 mg/mL 范围内促进 HepG2 细胞消耗培养基中的葡萄糖。在 0.5 mg/mL 时葡萄糖的相对消耗率（RCR）最高，超过了盐酸二甲双胍，以及相同浓度下的茶多糖。建立了有意义的降血糖多糖结构活性关系（SAR）。该结果表明 CCP 可作为降血糖药物或保健品使用。
