School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Grain and Oil Resources Comprehensive Exploitation and Engineering Technology Research Center of State of Administration of Grain, Wuhan 430023, China.
Mar Drugs. 2022 Feb 23;20(3):160. doi: 10.3390/md20030160.
Improved methods for the extraction of eicosapentaenoic acid (EPA), an essential and economically important polyunsaturated fatty acid, are urgently required. However, lipid extraction rates using food-grade solvents such as ethanol are usually low. To improve the ethanol-based extraction rate, and to elucidate the relevant mechanisms, we used cellulase and laccase to treat powdered , one of the most promising microalgal sources of EPA. Cellulase and laccase synergistically increased lipid yields by 69.31% and lipid EPA content by 42.63%, by degrading the amorphous hemicellulose and cellulose, improving crystallinity, and promoting the release and extraction of lysodiacylglyceryltrimethylhomoserine. Scanning electron microscopy showed that cell morphology was substantially altered, with cell-wall rupture, loss of cell boundaries, and the release of intracellular substances. In conclusion, lipid yields may be directly linked to cell-wall hemicellulose structure, and enzymatic treatment to alter this may improve lipid yields.
迫切需要改进二十碳五烯酸（EPA）的提取方法，EPA 是一种必需的、具有重要经济价值的多不饱和脂肪酸。然而，使用食品级溶剂（如乙醇）提取脂质的效率通常较低。为了提高基于乙醇的提取率，并阐明相关机制，我们使用纤维素酶和漆酶处理微藻粉，这是 EPA 的最有前途的微藻来源之一。纤维素酶和漆酶协同作用将脂质产率提高了 69.31%，将脂质 EPA 含量提高了 42.63%，这是通过降解无定形半纤维素和纤维素、提高结晶度以及促进溶血磷脂酰甘油三甲内盐的释放和提取来实现的。扫描电子显微镜显示，细胞形态发生了实质性变化，细胞壁破裂，细胞边界丧失，细胞内物质释放。总之，脂质产率可能与细胞壁半纤维素结构直接相关，通过酶处理改变这种结构可能会提高脂质产率。
