College of Biopharmaceutical and Food Engineering, Shangluo University, Shangluo 726000, China.
Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
Int J Biol Macromol. 2018 Aug;115:114-123. doi: 10.1016/j.ijbiomac.2018.04.049. Epub 2018 Apr 12.
This study was designed to investigate the chemical characterization and bioactivity of the Trametes lactinea (Berk.) Pat polysaccharides (TLP). The crude TLP was fractionated into two fractions, namely TLP-1 and TLP-2 with Cellulose DEAE-52 and Sephadex G-150. HPLC and FT-IR analysis showed that TLP-1 and TLP-2 were heteropolysaccharides mainly composed of glucose with the average molecular weights of 443.19kDa and 388.83kDa, respectively. TLP-1 from water elution possessed of higher reducing power and scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical and hydroxyl radical than TLP-2 eluted by 0.1M of NaCl. In comparison with TLP-2, TLP-1 showed stronger growth inhibition against human hepatoblastoma HepG-2 cells and caused higher LDH leakage. However, TLP-1 showed lower growth inhibition against normal hepatocyte L-02 cells and lower LDH leakage than TLP-2. Flow cytometric analysis showed that TLP-1 had a stimulatory effect on apoptosis of HepG-2 cells. These findings suggested that the polysaccharides, especially TLP-1 could contribute to the potential anticancer effects of T. lactinea (Berk.) Pat, which might be valuable as a natural antioxidant source applied in both healthy medicine and food industry for health benefits.
本研究旨在探究云芝（Trametes lactinea (Berk.) Pat）多糖（TLP）的化学特征和生物活性。粗 TLP 经纤维素 DEAE-52 和葡聚糖凝胶 G-150 柱层析分离为两个组分，即 TLP-1 和 TLP-2。HPLC 和 FT-IR 分析表明，TLP-1 和 TLP-2 均为主要由葡萄糖组成的杂多糖，其平均分子量分别为 443.19 kDa 和 388.83 kDa。从水相中洗脱的 TLP-1 具有较高的还原能力和对 1,1-二苯基-2-苦基肼（DPPH）自由基、超氧自由基和羟自由基的清除活性，而用 0.1M NaCl 洗脱的 TLP-2 则不然。与 TLP-2 相比，TLP-1 对人肝癌 HepG-2 细胞的生长抑制作用更强，导致 LDH 漏出量更高。然而，TLP-1 对正常肝细胞 L-02 的生长抑制作用和 LDH 漏出量均低于 TLP-2。流式细胞术分析表明，TLP-1 对 HepG-2 细胞的凋亡具有刺激作用。这些发现表明，多糖，特别是 TLP-1，可能有助于云芝（Trametes lactinea (Berk.) Pat）的潜在抗癌作用，这可能使其成为一种有价值的天然抗氧化剂来源，可应用于健康医学和食品工业，以促进健康。
