Bounegru Alexandra Virginia, Iticescu Catalina, Georgescu Lucian P, Apetrei Constantin
Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, "Dunărea de Jos" University of Galati, 47 Domneasca Street, 800008 Galați, Romania.
Int J Mol Sci. 2024 Apr 17;25(8):4419. doi: 10.3390/ijms25084419.
Phenolic compounds, originating from industrial, agricultural, and urban sources, can leach into flowing waters, adversely affecting aquatic life, biodiversity, and compromising the quality of drinking water, posing potential health hazards to humans. Thus, monitoring and mitigating the presence of phenolic compounds in flowing waters are essential for preserving ecosystem integrity and safeguarding public health. This study explores the development and performance of an innovative sensor based on screen-printed electrode (SPE) modified with graphene (GPH), poly(3,4-ethylenedioxythiophene) (PEDOT), and tyrosinase (Ty), designed for water analysis, focusing on the manufacturing process and the obtained electroanalytical results. The proposed biosensor (SPE/GPH/PEDOT/Ty) was designed to achieve a high level of precision and sensitivity, as well as to allow efficient analytical recoveries. Special attention was given to the manufacturing process and optimization of the modifying elements' composition. This study highlights the potential of the biosensor as an efficient and reliable solution for water analysis. Modification with graphene, the synthesis and electropolymerization deposition of the PEDOT polymer, and tyrosinase immobilization contributed to obtaining a high-performance and robust biosensor, presenting promising perspectives in monitoring the quality of the aquatic environment. Regarding the electroanalytical experimental results, the detection limits (LODs) obtained with this biosensor are extremely low for all phenolic compounds (8.63 × 10 M for catechol, 7.72 × 10 M for 3-methoxycatechol, and 9.56 × 10 M for 4-methylcatechol), emphasizing its ability to accurately measure even subtle variations in the trace compound parameters. The enhanced sensitivity of the biosensor facilitates detection and quantification in river water samples. Analytical recovery is also an essential aspect, and the biosensor presents consistent and reproducible results. This feature significantly improves the reliability and usefulness of the biosensor in practical applications, making it suitable for monitoring industrial or river water.
源自工业、农业和城市源的酚类化合物会渗入流动水体,对水生生物、生物多样性产生不利影响,并损害饮用水质量,对人类构成潜在健康危害。因此,监测和减轻流动水体中酚类化合物的存在对于维护生态系统完整性和保障公众健康至关重要。本研究探索了一种基于丝网印刷电极(SPE)的创新传感器的开发和性能,该电极用石墨烯(GPH)、聚(3,4-乙撑二氧噻吩)(PEDOT)和酪氨酸酶(Ty)进行了修饰,专为水分析设计,重点关注制造过程和获得的电分析结果。所提出的生物传感器(SPE/GPH/PEDOT/Ty)旨在实现高精度和高灵敏度,并实现高效的分析回收率。特别关注了制造过程以及修饰元素组成的优化。本研究突出了该生物传感器作为水分析高效可靠解决方案的潜力。用石墨烯进行修饰、PEDOT聚合物的合成和电聚合沉积以及酪氨酸酶的固定化有助于获得高性能且坚固的生物传感器,在监测水生环境质量方面展现出广阔前景。关于电分析实验结果,该生物传感器对所有酚类化合物获得的检测限(LOD)极低(儿茶酚为8.63×10⁻⁷ M,3-甲氧基儿茶酚为7.72×10⁻⁷ M,4-甲基儿茶酚为9.56×10⁻⁷ M),强调了其准确测量痕量化合物参数中即使细微变化的能力。生物传感器增强的灵敏度便于对河水样品进行检测和定量。分析回收率也是一个重要方面,该生物传感器呈现出一致且可重复的结果。这一特性显著提高了生物传感器在实际应用中的可靠性和实用性,使其适用于监测工业用水或河水。