Shanghai Key Laboratory of Functional Materials Chemistry & Department of Chemistry, East China University of Science and Technology, Shanghai, China.
Biosens Bioelectron. 2011 Mar 15;26(7):3181-6. doi: 10.1016/j.bios.2010.12.022. Epub 2010 Dec 30.
A highly efficient enzyme-based screen printed electrode (SPE) was obtained by using covalent attachment between 1-pyrenebutanoic acid, succinimidyl ester (PASE) adsorbing on the graphene oxide (GO) sheets and amines of tyrosinase-protected gold nanoparticles (Tyr-Au). Herein, the bi-functional molecule PASE was assembled onto GO sheets. Subsequently, the Tyr-Au was immobilized on the PASE-GO sheets forming a biocompatible nanocomposite, which was further coated onto the working electrode surface of the SPE. The characterization of obtained nanocomposite and modified SPE surface was investigated by atomic force microscopy (AFM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Attributing to the synergistic effect of GO-Au integration and the good biocompatibility of the hybrid-material, the fabricated disposable biosensor (Tyr-Au/PASE-GO/SPE) exhibited a rapid amperometric response (less than 6s) with a high sensitivity and good storage stability for monitoring catechol. This method shows a good linearity in the range from 8.3×10(-8) to 2.3×10(-5) M for catechol with a squared correlation coefficient of 0.9980, a quantitation limit of 8.2×10(-8) M (S/N=10) and a detection limit of 2.4×10(-8) M (S/N=3). The Michaelis-Menten constant was measured to be 0.027 mM. This disposable tyrosinase biosensor could offer a great potential for rapid, cost-effective and on-field analysis of phenolic compounds.
一种高效的基于酶的丝网印刷电极（SPE）是通过共价附着在氧化石墨烯（GO）片上的 1- 吡咯丁酸，琥珀酰亚胺酯（PASE）吸附和酪氨酸酶保护的金纳米粒子（Tyr-Au）的胺之间获得的。在此，双功能分子 PASE 被组装到 GO 片上。随后，Tyr-Au 固定在 PASE-GO 片上形成生物相容性纳米复合材料，进一步涂覆在 SPE 的工作电极表面上。通过原子力显微镜（AFM）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）研究了所获得的纳米复合材料和修饰 SPE 表面的特性。由于 GO-Au 集成的协同效应和混合材料的良好生物相容性，所制备的一次性生物传感器（Tyr-Au/PASE-GO/SPE）表现出快速的安培响应（小于 6s），具有高灵敏度和良好的储存稳定性，用于监测儿茶酚。该方法对儿茶酚的线性范围为 8.3×10(-8)至 2.3×10(-5) M，平方相关系数为 0.9980，定量限为 8.2×10(-8) M（S/N=10），检测限为 2.4×10(-8) M（S/N=3）。米氏常数为 0.027 mM。这种一次性酪氨酸酶生物传感器为快速、经济高效和现场分析酚类化合物提供了巨大的潜力。
