Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, E-32004 Ourense, Spain.
ANFACO-CECOPESCA, Carretera Colegio Universitario, 16, Vigo, Spain.
Meat Sci. 2018 Aug;142:38-43. doi: 10.1016/j.meatsci.2018.04.005. Epub 2018 Apr 9.
Cortisol and corticosterone in saliva were evaluated as pig stress biomarkers, using pig genotype (Duroc, L62 or Pietrain) and lairage time in the slaughterhouse (0, 2.0, 4.0 or 6.0 h) as controlled variables. Although some pigs were found to be carriers of stress susceptibility, all were healthy heterozygous individuals. Pre-slaughter transport increased cortisol levels in saliva above 3.0 μg/L (medium stress), and 4.0 h of lairage in the slaughterhouse raised them above 6.0 μg/L, whereas corticosterone concentrations exceeded 4 μg/L, which are suggestive of high stress. The highest cortisol levels were detected in the Duroc genotype. Other factors such as food deprivation, background noise, the presence of a large number of animals waiting to be slaughtered, mixing with unfamiliar animals or recent mixing of genders may also influence stress. Corticosterone proved a reliable indicator of high stress only. Meat quality from the pig breeds studied was not affected by lairage in the slaughterhouse for up to 6.0 h.
唾液中的皮质醇和皮质酮被评估为猪应激生物标志物，使用猪基因型（杜洛克、L62 或皮特兰）和屠宰场的待宰时间（0、2.0、4.0 或 6.0 小时）作为控制变量。尽管发现一些猪是应激易感性的携带者，但它们都是健康的杂合个体。宰前运输使唾液中的皮质醇水平升高至 3.0μg/L 以上（中度应激），而在屠宰场待宰 4.0 小时后皮质醇水平升高至 6.0μg/L 以上，而皮质酮浓度超过 4μg/L，则表明存在高度应激。杜洛克基因型的皮质醇水平最高。其他因素，如禁食、背景噪音、大量动物等待屠宰、与不熟悉的动物混合或最近的性别混合，也可能影响应激。皮质酮仅被证明是高度应激的可靠指标。在屠宰场待宰时间不超过 6.0 小时，不会影响研究猪品种的肉质。
