Qu Xuewei, Qiu Penghe, Zhu Ye, Yang Mingying, Mao Chuanbin
Stephenson Life Sciences Research Center, Department of Chemistry and Biochemistry, University of Oklahoma, Norman, OK, USA.
Institute of Applied Bioresource Research, College of Animal Science, Zhejiang University, Hangzhou, Zhejiang, China.
NPG Asia Mater. 2017;9. doi: 10.1038/am.2017.196. Epub 2017 Dec 8.
Precision medicine emphasizes patient-specific formulation for treatment of diseases, especially cancer. However, in targeted cancer treatment, because the expression level of tumor receptors in each patient varies even for the same type of cancer, the ligand/receptor-mediated approach does not seem promising for precision medicine. In this work, we demonstrated our strategy of using a phage display technique for breast cancer precision medicine. Using biopanning, we first selected an MCF-7 breast tumor-targeting peptide, then tested the effectiveness of the as-selected peptide in tumor homing and finally conjugated the peptide to a model photothermal drug, namely, gold nanorods, to achieve enhanced cancer killing efficacy. The peptides identified by the phage display technique can guide the drug to the tumors without the need to know the exact receptors on the tumor. This approach requires significantly less effort to explore patient-specific targeting molecules for precision medicine.
精准医学强调针对疾病，尤其是癌症的个体化治疗方案。然而，在靶向癌症治疗中，由于即使是同一类型的癌症，每个患者体内肿瘤受体的表达水平也存在差异，因此配体/受体介导的方法在精准医学中似乎前景不佳。在这项研究中，我们展示了利用噬菌体展示技术实现乳腺癌精准医学的策略。通过生物淘选，我们首先筛选出一种靶向MCF-7乳腺肿瘤的肽段，然后测试所筛选肽段在肿瘤归巢方面的有效性，最后将该肽段与一种模型光热药物（即金纳米棒）偶联，以提高癌症杀伤效果。通过噬菌体展示技术鉴定出的肽段能够引导药物靶向肿瘤，而无需了解肿瘤上的确切受体。这种方法在探索精准医学中针对患者的靶向分子时所需的工作量显著减少。
