Gene Expression Analysis of Two Epithelial-mesenchymal Transition-related Genes: Long Noncoding and in Gastric Cancer Tissues.

BACKGROUND

Cancer is the second cause of death after cardiovascular diseases worldwide. Tumor metastasis is the main cause of death in patients with cancer; therefore, unraveling the molecular mechanisms involved in metastasis is critical. Epithelial-mesenchymal transition (EMT) is believed to promote tumor metastasis. Based on the critical roles of long noncoding () and genes in cancer pathogenesis and EMT, in this study, we aimed to assess expression profile and clinicopathological relevance of these two genes in human gastric cancer.

MATERIALS AND METHODS

Quantitative real-time polymerase chain reaction was performed to assess these gene expressions in gastric cancer tissues and various cell lines. The associations between these gene expressions and clinicopathological characteristics were also analyzed.

RESULTS

Insignificant downregulation of and significant upregulation of in cancerous versus noncancerous gastric tissues were observed. Among different examined cell lines, all displayed both genes expression. Except for a significant inverse correlation between the expression levels of and depth of invasion (T) and a direct association between levels and advanced tumor grades, no significant association was found with other clinicopathological characteristics.

CONCLUSION

and genes may play a critical role in gastric cancer progression and may serve as potential diagnostic/prognostic biomarkers in cancer patients.